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. 2018:1740:59-67.
doi: 10.1007/978-1-4939-7652-2_6.

Isolation of Extracellular RNA from Bile

Irene K Yan  1 , Valentin X Berdah  2 , Tushar Patel  2
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Isolation of Extracellular RNA from Bile

Irene K Yan et al. Methods Mol Biol. 2018.

Abstract

The study of extracellular RNA has been recently reported as a tool for biomarker discovery. Extracellular vesicles can be isolated from different types of body fluids which contain protein, mRNA, and noncoding RNA. Extracellular RNA isolated from bile could be a useful tool for analyzing biliary tract diseases or cancer. Herein, we describe protocols based on modifications of commercially available kits for the collection, processing, and isolation of extracellular RNA from bile.

Keywords: Bile; Exosomes; Extracellular RNA; Extracellular Vesicles; Isolation.

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