Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2017 Sep-Dec;21(3):402-406.
doi: 10.4103/jomfp.JOMFP_207_17.

Immunofluorescence in oral lesions

S J Nithya  1 , R Sankarnarayanan  1 , V T Hemalatha  1 , T Sarumathi  1
Affiliations
Review

Immunofluorescence in oral lesions

S J Nithya et al. J Oral Maxillofac Pathol. 2017 Sep-Dec.

Abstract

Immunofluorescence is an immunohistochemical method used to demonstrate the presence of antigen and antibodies in the tissues or serum. It is an auxiliary diagnostic tool for the autoimmune bullous and inflammatory disorders. It detects the in situ and circulating immune deposits that are involved in the pathogenesis of immunobullous diseases.

Keywords: Immunofluorescence; immunobullous diseases; immunohistochemistry.

PubMed Disclaimer

Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
(a) Bullous pemphigoid. (b) Epidermolysis bullosa acquisita
Figure 2
Figure 2
Pemphigus vulgaris, linear intercellular C3, lower layers of the epithelium
Figure 3
Figure 3
Paraneoplastic pemphigus - linear, intercellular and intraepithelial deposition of IgG in the basement membrane
Figure 4
Figure 4
Bullous pemphigoid - linear C3 deposits in the basement membrane
Figure 5
Figure 5
EBA-Linear IgG deposits in the basement membrane
Figure 6
Figure 6
Linear IgA disease - IgA deposits in the basement membrane
Figure 7
Figure 7
Dermatitis herpetiformis - granulous IgA, top of the dermal papillae
Figure 8
Figure 8
Lupus erythematosus - continuous linear deposition of C3 along the basement membrane
Figure 9
Figure 9
Lichen planus
See this image and copyright information in PMC

References

    1. Robinson JP, Sturgis BS, Kumar GL. Immunofluorescence, IHC Staining Methods. 5th ed. Ch. 10. Science and Education Publishing; 2014. pp. 61–5.
    1. Premalatha BR, Roopa SR, Vijaya M. Immunofluorescence in oral pathology: Part I-methodology. World J Dent. 2011;2:326–33.
    1. Arvind Babu RS, Chandrasekar P, Lalith P, Sridhar Reddy CK, Kiran Kumar K, Ramana Reddy BV. Immunofluorescence and its application in dermatopathology with oral manifestations. Rev J OrofacSci. 2013;5:2–8.
    1. Coons AH, Creech HJ, Jones RN, Berliner E. The demonstration of pneumococcal antigen in tissues by the use of fluorescent antibody. J Immunol. 1942;45:159–70.
    1. Mohan PH, Pai S, Rao R, Sripathi R, Smitha P. Technique of immunofluorescence and their significance. Indian J Dermatol VenerLeprol. 2008;74:415–9. - PubMed