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. 2013 Feb;3(1):20-27.
doi: 10.1016/j.jpha.2012.07.008. Epub 2012 Jul 27.

Study on changes of polyamine levels in mice with the development of U14 cervical cancer

Qian Wang  1 , Yi-Xiang Wang  2 , Ran Liu  1 , Yan Zhou  1 , Ying Jia  2 , Xiang-Lin Wang  1 , Yu Hu  3 , Kai-Shun Bi  1 , Qing Li  1
Affiliations

Study on changes of polyamine levels in mice with the development of U14 cervical cancer

Qian Wang et al. J Pharm Anal. 2013 Feb.

Abstract

This study was performed to investigate the possible involvement of polyamines in the development of cervical cancer. The objective of the present study was therefore to find the specific polyamine indicators, which could be used as useful markers for the early determination of cervical cancer. A simple method for the simultaneous determination of plasma concentrations of five polyamines in normal and U14 model mice was developed by using HPLC-MS. The samples were derivatized by benzoyl chloride. The derived polyamines were separated on a C18 column by a gradient elution with methanol-water, and then detected with HPLC-MS. The results showed that all polyamine levels in the U14 model mice were higher than those in normal ones. The cadaverine, putrescine and 1, 3-diaminopropane levels were significantly higher in U14 model mice plasma than those in normal mice plasma, especially the putrescine and 1, 3-diaminopropane (P<0.01). The cadaverine, putrescine and 1, 3-diaminopropane levels were significantly higher in U14 mice model urine than those in normal mice urine, especially the cadaverine and 1, 3-diaminopropane (P<0.01). Putrescine, cadaverine and 1, 3-diaminopropane might be the indicators of the cervical cancer.

Keywords: Cervical cancer; HPLC-MS; Mice; Plasma; Polyamines; Urine.

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Figures

Fig. 1
Fig. 1
Total ion chromatograms (TIC) obtained from standard polyamines (A), plasma sample (B) and urine sample (C) by HPLC-ESI-MS. (1) PUT, (2) DAP, (3) CAD, (4) DHA (I.S.), (5) SPD, and (6) SPM.
Fig. 2
Fig. 2
Result of the cluster analysis from mice plasma samples, the Between-groups linkage method was adopted and squared Euclidean distance was chosen as measurement. Sixteen samples were clustered into two groups, i.e. Group I and Group II. Bar=5 rescaled distance.
Fig. 3
Fig. 3
Result of the cluster analysis from mice urine samples, the Between-groups linkage method was adopted and squared Euclidean distance was chosen as measurement. Eight samples were clustered into two groups, i.e. Group I and Group II. Bar=5 rescaled distance.
Fig. 4
Fig. 4
The possible metabolic process of polyamines. ODC, Ornithine decarboxylase, SAMDC, S-adenosylmethionine decarboxylase.
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