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. 2013 Oct;3(5):341-348.
doi: 10.1016/j.jpha.2013.03.005. Epub 2013 Apr 2.

Application of LC-MS/MS for quantitative analysis of glucocorticoids and stimulants in biological fluids

Jamshed Haneef  1 , Mohammad Shaharyar  1 , Asif Husain  1 , Mohd Rashid  1 , Ravinesh Mishra  1 , Shama Parveen  1 , Niyaz Ahmed  1 , Manoj Pal  2 , Deepak Kumar  3
Affiliations

Application of LC-MS/MS for quantitative analysis of glucocorticoids and stimulants in biological fluids

Jamshed Haneef et al. J Pharm Anal. 2013 Oct.

Abstract

Liquid chromatography tandem mass chromatography (LC-MS/MS) is an important hyphenated technique for quantitative analysis of drugs in biological fluids. Because of high sensitivity and selectivity, LC-MS/MS has been used for pharmacokinetic studies, metabolites identification in the plasma and urine. This manuscript gives comprehensive analytical review, focusing on chromatographic separation approaches (column packing materials, column length and mobile phase) as well as different acquisition modes (SIM, MRM) for quantitative analysis of glucocorticoids and stimulants. This review is not meant to be exhaustive but rather to provide a general overview for detection and confirmation of target drugs using LC-MS/MS and thus useful in the doping analysis, toxicological studies as well as in pharmaceutical analysis.

Keywords: Biological fluid; Glucocorticoids; Hyphenated techniques; Ionization techniques; LC–MS/MS; Stimulants.

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Figures

Fig. 1
Fig. 1
Proposed mass fragmentation pathway of dexamethasone drug. 1—Molecular ion peak at M+=393. 2—Base ion peak at m/z=373. 3—Daughter ion peak at m/z=355.
Fig. 2
Fig. 2
Proposed mass fragmentation pathway of prednisolone drug. 1—Molecular ion peak at M+=361. 2—Base ion peak at m/z=343. 3—Daughter ion peak at m/z=307.
Fig. 3
Fig. 3
Proposed mass fragmentation pathway of prednisone drug. 1—Molecular ion peak at M+=359. 2—Base ion peak at m/z=341. 3—Daughter ion peak at m/z=171.
Fig. 4
Fig. 4
Proposed mass fragmentation pathway of strychnine drug. 1—Molecular ion peak at M+=335. 2—Base ion peak at m/z=264. 3—Daughter ion peak at m/z=156.
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