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. 2013 Oct;3(5):360-366.
doi: 10.1016/j.jpha.2012.12.011. Epub 2013 Jan 11.

A novel luminol-based chemiluminescence method for the determination of amikacin sulfate in serum by using trivalent copper-periodate complex

Yu-Fei Hu  1   2 , Gong-Ke Li  1 , Zhu-Jun Zhang  2
Affiliations

A novel luminol-based chemiluminescence method for the determination of amikacin sulfate in serum by using trivalent copper-periodate complex

Yu-Fei Hu et al. J Pharm Anal. 2013 Oct.

Abstract

A novel chemiluminescence (CL) reaction was based on the oxidizing reaction of luminol by the trivalent copper-periodate complex (K5[Cu(HIO6)2], DPC) in alkaline medium. The CL intensity could be enhanced in the presence of amikacin sulfate (AKS). A new CL method was developed for the determination of AKS by coupling with flow injection (FI) technology. Because of the distinctive oxidative effect of DPC, the luminol-based CL reaction could occur at a low concentration of 10-7 M. The relative CL intensity was proportional to the concentration of AKS in the range of 4.0×10-9-4.0×10-6 g/mL with the detection limit of 1.2×10-9 g/mL. The relative standard deviation was 2.1% for 8.0×10-9 g/mL AKS (n=9). The proposed method was successfully applied to the direct determination of AKS at the level of ng/mL in serum samples. The recovery varied from 97.0% to 106.3%. A possible mechanism of the CL reaction was discussed in detail by relating to the CL kinetic characteristics and electrochemical activities of the oxidant DPC.

Keywords: Amikacin sulfate; Chemiluminescence; Flow injection; K5[Cu(HIO6)2]; Serum sample.

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Figures

Scheme 1
Scheme 1
Structure of amikacin sulfate.
Fig. 1
Fig. 1
Schematic diagram of the CL-FIA system. P: peristaltic pump; V: injection valve; M: mixtured Position; F: spiral glass flow cell; PMT: photomultiplier tube; W: waste; H: light tight house; PC: personal computer. (a) luminol solution; (b) analyte solution; (c) DPC solution.
Fig. 2
Fig. 2
Kinetics curves of luminol-DPC catalyzed by samples. Luminol solution, 1×10−7 M; DPC solution, 4×10−4 M; injected AKS solution, 2×10−7 g/mL.
Fig. 3
Fig. 3
The effect of the luminol concentration. DPC solution, 4×10−4 M; AKS solution, 4×10−9 g/mL; flow rate, 2.0 mL/min.
Fig. 4
Fig. 4
The effect of the DPC concentration. Luminol solution, 1×10−7 M; AKS solution, 4×10−9 g/mL; flow rate, 2.0 mL/min.
Fig. 5
Fig. 5
The effect of medium pH of luminol solution. Luminol solution, 1×10−7 M; DPC solution, 4×10−4 M; AKS solution, 4×10−9 g/mL; flow rate, 2.0 mL/min.
Fig. 6
Fig. 6
CL spectrum of the luminol-DPC-licomycin system. DPC, 1.0 × 10−4 M; luminol, 1× 10−4 M; AKS, 2.0 × 10−4 g/mL; 1, in the absence of AKS; 2, in the presence of AKS.
Fig. 7
Fig. 7
One reaction mechanism suggested for the CL reaction between DPC and luminol.
None
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