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. 2014 Feb;4(1):6-13.
doi: 10.1016/j.jpha.2013.03.002. Epub 2013 Apr 11.

Metabolic profiling of plasma from cardiac surgical patients concurrently administered with tranexamic acid: DI-SPME-LC-MS analysis

Barbara Bojko  1 , Marcin Wąsowicz  2 , Janusz Pawliszyn  1
Affiliations

Metabolic profiling of plasma from cardiac surgical patients concurrently administered with tranexamic acid: DI-SPME-LC-MS analysis

Barbara Bojko et al. J Pharm Anal. 2014 Feb.

Abstract

A metabolic profile of plasma samples from patients undergoing heart surgery with the use of cardiopulmonary bypass (CPB) and concurrent administration of tranexamic acid was determined. Direct immersion solid phase microextraction (DI-SPME), a new sampling and sample preparation tool for metabolomics, was used in this study for the first time to investigate clinical samples. The results showed alteration of diverse compounds involved in different biochemical pathways. The most significant contribution in changes induced by surgery and applied pharmacotherapy was noticed in metabolic profile of lysophospholipids, triacylglycerols, mediators of platelet aggregation, and linoleic acid metabolites. Two cases of individual response to treatment were also reported.

Keywords: Cardiopulmonary bypass; Direct immersion solid phase microextraction; Heart surgery; LC/MS; Metabolomics; Tranexamic acid.

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Figures

Fig. 1
Fig. 1
Principal component analysis for PC1, PC2 and PC3. Good clustering of blank and QC samples and the existence of outliers can be observed.
Fig. 2
Fig. 2
PLS-DA score plot showing the separation of two cohorts (group of patients before and during surgery and drug administration).
Fig. 3
Fig. 3
Loading plots for components 1 and 2 (A) and 2 and 3 (B) showing variables differentiating dosed and non-dosed group of patients.
Fig. 4
Fig. 4
Individual changes in response to surgery and drug administration. Distribution of m/z 315.25 (A) and 450.32 (B) variables among the studied group of patients shows significant increase of their level in plasma samples of patient 9D and patient 7D, respectively.
None
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