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Review
. 2015 Apr;5(2):75-84.
doi: 10.1016/j.jpha.2014.06.002. Epub 2014 Jun 26.

Bioautography and its scope in the field of natural product chemistry

Saikat Dewanjee  1 , Moumita Gangopadhyay  2 , Niloy Bhattacharya  1 , Ritu Khanra  1 , Tarun K Dua  1
Affiliations
Review

Bioautography and its scope in the field of natural product chemistry

Saikat Dewanjee et al. J Pharm Anal. 2015 Apr.

Abstract

Medicinal plants, vegetables and fruits are the sources of huge number of bioactive lead/scaffolds with therapeutic and nutraceutical importance. Bioautography is a means of target-directed isolation of active molecules on chromatogram. Organic solvents employed in chromatographic separation process can be completely removed before biological detection because these solvents cause inactivation of enzymes and/or death of living organisms. They offer a rapid and easy identification of bioactive lead/scaffolds in complex matrices of plant extracts. Bioautography is a technique to isolate hit(s)/lead(s) by employing a suitable chromatographic process followed by a biological detection system. This review critically describes the methodologies to identify antimicrobial, antioxidant, enzyme inhibitor lead/scaffolds by employing bioautography. A significant number of examples have been incorporated to authenticate the methodologies.

Keywords: Bioactivity; Bioassay; Bioautography; Detection principle; Thin layer chromatography.

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Figures

Fig. 1
Fig. 1
Schematic overview of contact bioautography.
Fig. 2
Fig. 2
Schematic diagram indicates the direct bioautographic process.
Fig. 3
Fig. 3
Schematic diagram of immersion or agar overlay bioautography.
Fig. 4
Fig. 4
Reduction of tetrazolium salts.
Fig. 5
Fig. 5
Reaction of DPPH radical with radical scavengers.
Fig. 6
Fig. 6
Reaction of ABTS radical with radical scavengers.
Fig. 7
Fig. 7
Schematic diagram of inhibition of bleaching of β-carotene.
Fig. 8
Fig. 8
Reaction between acetylcholine esterase with α-napthyl acetate, produced α-napthol again reacts with fast blue B salt to produce purple colored azo dye.
Fig. 9
Fig. 9
Ellman reaction.
Fig. 10
Fig. 10
Diazotization reaction for the detection of α and β-glucosidase inhibition.
Fig. 11
Fig. 11
Reaction to detect xanthine oxidase inhibitors.
Fig. 12
Fig. 12
Schematic diagram of the HPTLC-YES process.
See this image and copyright information in PMC

References

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