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. 2015 Dec;5(6):378-382.
doi: 10.1016/j.jpha.2014.11.002. Epub 2014 Nov 29.

Antimicrobial and antiproliferative prospective of kosinostatin - a secondary metabolite isolated from Streptomyces sp

Vinayagam Rambabu  1 , Subramaniyan Suba  2 , Suburamaniyan Vijayakumar  1
Affiliations

Antimicrobial and antiproliferative prospective of kosinostatin - a secondary metabolite isolated from Streptomyces sp

Vinayagam Rambabu et al. J Pharm Anal. 2015 Dec.

Abstract

Cancer is a communal health hazard worldwide. The present investigation attempts to evaluate antimicrobial and anticancer potential of kosinostatin on mammary carcinoma cell line (MCF-7). The anticancer and antiproliferative activities of kosinostatin were analyzed on MCF cell line by MTT assay and cytotoxicity assays like lactate dehydrogenase (LDH) and glutathione (GSH). The secondary metabolite kosinostatin exhibited its apoptotic nature by expressing p53 protein. Collectively, the results acquired from this study promise that kosinostatin shows the potent anticancer activity.

Keywords: Cytotoxic activity; Kosinostatin; MCF-7; Secondary metabolite; Streptomyces sp..

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Figures

Fig. 1
Fig. 1
The chemical structure of kosinostatin (Quinocycline B).
Fig. 2
Fig. 2
Determination of minimum inhibitory concentration (MIC).
Fig. 3
Fig. 3
MTT cell viability assay on control and kosinostatin treated MCF-7 cell line.
Fig. 4
Fig. 4
Level of LDH leakage in control and kosinostatin treated MCF-7 cell lines.
Fig. 5
Fig. 5
Level of GSH in control and kosinostatin treated MCF-7 cells.
Fig. 6
Fig. 6
Western blotting analysis of control and kosinostatin treated MCF-7 cells.
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