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. 2018 Apr:254:31-34.
doi: 10.1016/j.jviromet.2018.01.009.

Evaluation of the inactivation of Venezuelan equine encephalitis virus by several common methods

Edward I Patterson  1 , Kelsey L Warmbrod  2 , Donald H Bouyer  2 , Naomi L Forrester  2
Affiliations

Evaluation of the inactivation of Venezuelan equine encephalitis virus by several common methods

Edward I Patterson et al. J Virol Methods. 2018 Apr.

Abstract

Working with virological samples requires validated inactivation protocols for safe handling and disposal. Although many techniques exist to inactivate samples containing viruses, not all procedures have been properly validated or are compatible with subsequent assays. To aid in the development of inactivation protocols for Alphaviruses, and specifically Venezuelan equine encephalitis virus (VEEV), a variety of methods were evaluated for their ability to completely inactivate a high titer sample of the vaccine strain VEEV TC-83. The methods evaluated include reagents used in RNA extraction, fixation, treatment with a detergent, and heat inactivation. Most methods were successful at inactivating the sample; however, treatment with only Buffer AVL, SDS, and heat inactivation at 58 °C for one hour were not capable of complete inactivation of the virus in the sample. These results provide a substantial framework for identifying techniques that are safe for complete inactivation of Alphaviruses and to advise protocol implementation.

Keywords: Alphavirus; Venezuelan equine encephalitis virus; Virus inactivation.

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Figures

Figure 1
Figure 1
The virus titer, and proportion of cytopathic effect (CPE) in each condition. A) The titer of virus produced in samples from media and Vero cells from infections with an MOI of 1 for 24 hours. B) The proportion of flasks with visible CPE from each condition. Control samples include 5 untreated, dialyzed samples serially diluted from 1 × 105 pfu to 1 × 101 pfu.
See this image and copyright information in PMC

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