Simultaneous demonstration of 3 synaptic markers: nerve endings, localization of AChE and AChR clusters at neuromuscular junctions of the rat in vitro
- PMID: 2941103
- DOI: 10.1111/j.1768-322x.1986.tb00451.x
Simultaneous demonstration of 3 synaptic markers: nerve endings, localization of AChE and AChR clusters at neuromuscular junctions of the rat in vitro
Abstract
Our triple labelling method allows the AChE and AChR distributions and the nerve endings to be stained in the same preparation. This method which provides a detailed visualization of the synaptic contacts and is capable of revealing a weak AChE activity is particularly valuable in studies of synaptogenesis.
Similar articles
-
Simultaneous visualization of acetylcholinesterase activity and acetylcholine receptor clusters at neuromuscular synapses in vivo and in vitro.Biol Cell. 1984;50(1):99-102. doi: 10.1111/j.1768-322x.1984.tb00255.x. Biol Cell. 1984. PMID: 6234043
-
Presynaptic or postsynaptic origin of acetylcholinesterase at neuromuscular junctions? An immunological study in heterologous nerve-muscle cultures.Dev Biol. 1986 Jul;116(1):69-77. doi: 10.1016/0012-1606(86)90044-8. Dev Biol. 1986. PMID: 3525279
-
Acetylcholinesterase from the motor nerve terminal accumulates on the synaptic basal lamina of the myofiber.J Cell Biol. 1991 Nov;115(3):755-64. doi: 10.1083/jcb.115.3.755. J Cell Biol. 1991. PMID: 1918162 Free PMC article.
-
Accumulation of acetylcholine receptors and acetylcholinesterase at newly formed nerve-muscle synapses.Pharmacol Rev. 1978 Dec;30(4):411-28. Pharmacol Rev. 1978. PMID: 392537 Review.
-
Significance of acetylcholinesterase in central synaptic transmission.Fed Proc. 1969 Jan-Feb;28(1):95-100. Fed Proc. 1969. PMID: 4303455 Review. No abstract available.
Cited by
-
Effects of electroacupuncture on recovery of the electrophysiological properties of the rabbit gastrocnemius after contusion: an in vivo animal study.BMC Complement Altern Med. 2015 Mar 19;15:69. doi: 10.1186/s12906-015-0601-z. BMC Complement Altern Med. 2015. PMID: 25887510 Free PMC article.
-
In Vitro Innervation as an Experimental Model to Study the Expression and Functions of Acetylcholinesterase and Agrin in Human Skeletal Muscle.Molecules. 2017 Aug 27;22(9):1418. doi: 10.3390/molecules22091418. Molecules. 2017. PMID: 28846617 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources