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. 2018 Mar;113(3):185-196.
doi: 10.1590/0074-02760170407.

Feline sporotrichosis: associations between clinical-epidemiological profiles and phenotypic-genotypic characteristics of the etiological agents in the Rio de Janeiro epizootic area

Jéssica Sepulveda Boechat  1 , Manoel Marques Evangelista Oliveira  2 , Rodrigo Almeida-Paes  2 , Isabella Dib Ferreira Gremião  1 , Ana Caroline de Sá Machado  1 , Raquel de Vasconcelos Carvalhaes Oliveira  3 , Anna Barreto Fernandes Figueiredo  1 , Vanessa Brito de Souza Rabello  2 , Karoline Benevides de Lima Silva  1 , Rosely Maria Zancopé-Oliveira  2 , Tânia Maria Pacheco Schubach  1 , Sandro Antonio Pereira  1
Affiliations

Feline sporotrichosis: associations between clinical-epidemiological profiles and phenotypic-genotypic characteristics of the etiological agents in the Rio de Janeiro epizootic area

Jéssica Sepulveda Boechat et al. Mem Inst Oswaldo Cruz. 2018 Mar.

Abstract

Background: Sporotrichosis is caused by species of the genus Sporothrix. From 1998 to 2015, 4,703 cats were diagnosed at the Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil. Even after the description of the Sporothrix species, the characterisation of feline isolates is not performed routinely.

Objectives: To characterise the clinical isolates from cats at the species level and correlate them with the clinical and epidemiological characteristics of the cats.

Methods: Forty seven Sporothrix spp. isolates from cats assisted at Fiocruz from 2010 to 2011 were included. Medical records were consulted to obtain the clinical and epidemiological data. The isolates were identified through their morphological and physiological characteristics. T3B polymerase chain reaction (PCR) fingerprinting was used for molecular identification of the species.

Findings: In phenotypic tests, 34 isolates were characterised as S. brasiliensis, one as S. schenckii and 12 as Sporothrix spp. PCR identified all isolates as S. brasiliensis.

Main conclusions: S. brasiliensis is the only etiological agent of feline sporotrichosis in Rio de Janeiro to date. None association was found between the isolates and the clinical and epidemiological data. In addition, we strongly recommend the use of molecular techniques for the identification of isolates of Sporothrix spp.

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Figures

Fig. 1
Fig. 1. geographic distribution of 47 cases of feline sporotrichosis assisted at the Laboratory of Clinical Research on Dermatozoonosis in Domestic Animals (INI)/Fiocruz, Rio de Janeiro - Brazil, 2010 to 2011.
Fig. 2
Fig. 2. dendogram demonstrating the degree of similarity between the profiles from the T3B polymerase chain reaction fingerprinting of the 47 feline isolates characterised as Sporothrix brasiliensis at the Laboratory of Clinical Research on Dermatozoonosis in Domestic Animals (INI)/Fiocruz, Rio de Janeiro - Brazil, 2010 to 2011.
Fig. 3
Fig. 3. clinical, epidemiological and phenotypic and molecular characterisation of the 47 isolates of the cats assisted at the Laboratory of Clinical Research on Dermatozoonosis in Domestic Animals (INI)/Fiocruz, Rio de Janeiro - Brazil, 2010 to 2011.
Fig. 4
Fig. 4. association between groups with and without discordant phenotype compared to molecular characterisation, and the treatment time to favorable outcome (in weeks) of cats assisted at the Laboratory of Clinical Research on Dermatozoonosis in Domestic Animals (INI)/Fiocruz, Rio de Janeiro - Brazil, 2010 to 2011.
See this image and copyright information in PMC

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