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. 2018 Jan;15(1):727-730.
doi: 10.3892/ol.2017.7395. Epub 2017 Nov 13.

Expression of microRNA-184 in glioma

Xiao-Ben Wu  1 , Wei Yang  2 , Gang Fan  1 , Wan-Run Lin  3 , Fang Liu  1 , Zhi-Ming Lu  1
Affiliations

Expression of microRNA-184 in glioma

Xiao-Ben Wu et al. Oncol Lett. 2018 Jan.

Abstract

The aim of the present study was to examine the expression of microRNA (miRNA)-184 in gliomas with different pathological grades, and its effect on survival prognosis. For the present study, 40 participants were selected with different pathological grades of glioma tissues with grade I (n=10), grade II (n=8), grade III (n=16), and grade IV (n=6). In addition, 10 cases of normal brain tissue (obtained by decompression because of traumatic brain injury) were selected. RT-PCR and immunohistochemical techniques were used to detect the expression level and intensity of miRNA-184 in different grades of glioma tissues. The length of survival of miRNA-184-positive patients was analyzed. miRNA-184 mRNA expression was found in normal tissues and tumor tissues, and the expression in tumor tissues was significant (P<0.05). Statistically significant differences of miRNA-184 expression were observed among different grades (P<0.05). miRNA-184 expression increased with the increase of grade level. The differences in expression across grade levels was statistically significant (P<0.05). A positive expression was not related to the pathological types of glioma cells. The median survival time of patients with miRNA-184-positive expression was significantly shorter than that of the negative expression group (P<0.05). miRNA-184 is highly expressed in gliomas, which is positively correlated with pathological grade, and is not correlated with pathological type, and negatively correlated with survival time. Thus, miRNA-184 is a potentially important molecular marker for glioma.

Keywords: RT-PCR method; glioma; immunohistochemistry; microRNA-184; pathological grade; survival time.

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Figures

Figure 1.
Figure 1.
RT-PCR was used to detect the expression level of miRNA-184 mRNA. *P<0.05.
Figure 2.
Figure 2.
Immunohistochemical staining of microRNA-184-positive expression (magnification, ×40, from left to right normal tissue, glioma grade I, II, III and IV).
Figure 3.
Figure 3.
Kaplan-Meier analysis of survival time (green line, microRNA-184- positive expression group; purple line, microRNA-184-negative expression group).
See this image and copyright information in PMC

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