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. 2018 Feb;8(2):114.
doi: 10.1007/s13205-018-1125-2. Epub 2018 Feb 6.

Complete genome sequence of Bacillus velezensis 157 isolated from Eucommia ulmoides with pathogenic bacteria inhibiting and lignocellulolytic enzymes production by SSF

Long Chen  1 , Wei Gu  2 , Hai-Yan Xu  2 , Gui-Lian Yang  1   3   4 , Xiao-Feng Shan  1 , Guang Chen  5 , Chun-Feng Wang  1   3   4 , Ai-Dong Qian  1   3   4
Affiliations

Complete genome sequence of Bacillus velezensis 157 isolated from Eucommia ulmoides with pathogenic bacteria inhibiting and lignocellulolytic enzymes production by SSF

Long Chen et al. 3 Biotech. 2018 Feb.

Abstract

Bacillus velezensis 157 was isolated from the bark of Eucommia ulmoides, and exhibited antagonistic activity against a broad spectrum of pathogenic bacteria and fungi. Moreover, B. velezensis 157 also showed various lignocellulolytic activities including cellulase, xylanase, α-amylase, and pectinase, which had the ability of using the agro-industrial waste (soybean meal, wheat bran, sugarcane bagasse, wheat straw, rice husk, maize flour and maize straw) under solid-state fermentation and obtained several industrially valuable enzymes. Soybean meal appeared to be the most efficient substrate for the single fermentation of B. velezensis 157. Highest yield of pectinase (19.15 ± 2.66 U g-1), cellulase (46.69 ± 1.19 U g-1) and amylase (2097.18 ± 15.28 U g-1) was achieved on untreated soybean meal. Highest yield of xylanase (22.35 ± 2.24 U g-1) was obtained on untreated wheat bran. Here, we report the complete genome sequence of the B. velezensis 157, composed of a circular 4,013,317 bp chromosome with 3789 coding genes and a G + C content of 46.41%, one circular 8439 bp plasmid and a G + C content of 40.32%. The genome contained a total of 8 candidate gene clusters (bacillaene, difficidin, macrolactin, butirosin, bacillibactin, bacilysin, fengycin and surfactin), and dedicates over 15.8% of the whole genome to synthesize secondary metabolite biosynthesis. In addition, the genes encoding enzymes involved in degradation of cellulose, xylan, lignin, starch, mannan, galactoside and arabinan were found in the B. velezensis 157 genome. Thus, the study of B. velezensis 157 broadened that B. velezensis can not only be used as biocontrol agents, but also has potentially a wide range of applications in lignocellulosic biomass conversion.

Keywords: Agro-industrial waste; Bacillus velezensis 157; Complete genome sequencing; Eucommia ulmoides; Lignocellulolytic enzyme activities; Secondary metabolites.

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Conflict of interest statement

Compliance with ethical standardsThe authors declare no conflict of interest.

Figures

Fig. 1
Fig. 1
Phylogenetic tree based on 16S rRNA gene sequences showing the relationship between Bacillus velezensis 157 and closely related Bacillus species. Listeria monocytogenes AMDK2 was used as an out-group. Bootstrap values (%) based on 1000 replications are given at nodes
Fig. 2
Fig. 2
The whole genome of Bacillus velezensis 157. The circular genome map consists of 7 circles. From the outer circle inward, each circle displays information about the genome of (1) forward CDS, (2) reverse CDS, (3) forward COG function classification, (4) reverse COG function classification, (5) nomenclature and locations of predictive secondary metabolite clusters, (6) G + C content and (7) GC Skew
See this image and copyright information in PMC

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