Characterisation of the simultaneous molybdenum reduction and glyphosate degradation by Burkholderia vietnamiensis AQ5-12 and Burkholderia sp. AQ5-13

Abstract

In this novel study, we report on the use of two molybdenum-reducing bacteria with the ability to utilise the herbicide glyphosate as the phosphorus source. The bacteria reduced sodium molybdate to molybdenum blue (Mo-blue), a colloidal and insoluble product, which is less toxic. The characterisation of the molybdenum-reducing bacteria was carried out using resting cells immersed in low-phosphate molybdenum media. Two glyphosate-degrading bacteria, namely Burkholderia vietnamiensis AQ5-12 and Burkholderia sp. AQ5-13, were able to use glyphosate as a phosphorous source to support molybdenum reduction to Mo-blue. The bacteria optimally reduced molybdenum between the pHs of 6.25 and 8. The optimum concentrations of molybdate for strain Burkholderia vietnamiensis strain AQ5-12 was observed to be between 40 and 60 mM, while for Burkholderia sp. AQ5-13, the optimum molybdate concentration occurred between 40 and 50 mM. Furthermore, 5 mM of phosphate was seen as the optimum concentration supporting molybdenum reduction for both bacteria. The optimum temperature aiding Mo-blue formation ranged from 30 to 40 °C for Burkholderia vietnamiensis strain AQ5-12, whereas for Burkholderia sp. AQ5-13, the range was from 35 to 40 °C. Glucose was the best electron donor for supporting molybdate reduction, followed by sucrose, fructose and galactose for both strains. Ammonium sulphate was the best nitrogen source in supporting molybdenum reduction. Interestingly, increasing the glyphosate concentrations beyond 100 and 300 ppm for Burkholderia vietnamiensis strain AQ5-12 and Burkholderia sp. AQ5-13, respectively, significantly inhibited molybdenum reduction. The ability of these bacteria to reduce molybdenum while degrading glyphosate is a useful process for the bioremediation of both toxicants.

Keywords: Burkholderia sp.; Burkholderia vietnamiensis; Glyphosate degrading; Molybdenum; Molybdenum blue.

Fig. 1

Effect of different carbon sources on Mo reduction by resting cells of Burkholderia vietnamiensis strain AQ5-12 and Burkholderia sp. strain AQ5-13. Data represent mean ± SD (n = 3)

Fig. 2

Effect of different Mo concentrations on reduction ability by resting cells of Burkholderia vietnamiensis strain AQ5-12 and Burkholderia sp. strain AQ5-13. Data represent mean ± SD (n = 3)

Fig. 3

Effect of different phosphate concentrations on Mo reduction by resting cells of Burkholderia vietnamiensis strain AQ5-12 and Burkholderia sp. strain AQ5-13. Data represent mean ± SD (n = 3)

Fig. 4

Effect of different pH level on molybdenum reduction by resting cells of Burkholderia vietnamiensis strain AQ5-12 and Burkholderia sp. strain AQ5-13 (n = 3)

Fig. 5

Effect of temperature on Mo reduction by resting cells of Burkholderia vietnamiensis strain AQ5-12 and Burkholderia sp. strain AQ5-13. Data represent mean ± SD (n = 3)

Fig. 6

Effect of nitrogen sources on Mo reduction by resting cells of Burkholderia vietnamiensis strain AQ5-12 and Burkholderia sp. strain AQ5-13. Data represent mean ± SD (n = 3)

Fig. 7

Effect of different glyphosate concentrations on Mo reduction by resting cells of Burkholderia vietnamiensis strain AQ5-12 and Burkholderia sp. strain AQ5-13. Data represent mean ± SD (n = 3)