Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Feb;15(2):2287-2295.
doi: 10.3892/ol.2017.7566. Epub 2017 Dec 8.

β-catenin, leucine-rich repeat-containing G protein-coupled receptor 5 and GATA-binding factor 6 are associated with the normal mucosa-adenoma-adenocarcinoma sequence of colorectal tumorigenesis

Bin Yang  1 , Liang Mao  1 , Yan Li  1 , Qia Li  2 , Xioli Li  1 , Yunjing Zhang  1 , Zhenhua Zhai  1   2
Affiliations

β-catenin, leucine-rich repeat-containing G protein-coupled receptor 5 and GATA-binding factor 6 are associated with the normal mucosa-adenoma-adenocarcinoma sequence of colorectal tumorigenesis

Bin Yang et al. Oncol Lett. 2018 Feb.

Abstract

In the present study, the expression of β-catenin, leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) and GATA6 was investigated during the transition from normal mucosa through to adenoma and adenocarcinoma in colorectal tissue sections obtained from 65 patients with a pathological diagnosis of colorectal adenocarcinoma and a history of adenoma. Immunohistochemical staining of β-catenin, LGR5 and GATA6 was performed and evaluated. The nuclear expression of β-catenin and the cytoplasmic expression of LGR5 and GATA6 were increased in samples as they progressed from normal mucosa to adenoma and adenocarcinoma. However, membrane-bound β-catenin and nuclear GATA6 expression decreased. Positive correlations were observed between the expression of LGR5 and cytoplasmic GATA6 in adenoma (P=0.0005; rs=0.48) and adenocarcinoma samples (P=0.007; rs=0.38): However, no significant association was observed in normal mucosa (P=0.399). The expression of nuclear β-catenin was significantly increased in the serosal layer compared with the invasive layers of the colorectal wall in samples of adenocarcinoma (P=0.042). The results of the present study suggest that the nuclear expression of β-catenin and LGR5 and the cytoplasmic expression of GATA6 function together during the development of colorectal carcinoma.

Keywords: GATA6; colorectal cancer; leucine-rich repeat-containing G protein-coupled receptor 5; tumorigenesis; β-catenin.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
The expression and intracellular localization of β-catenin, LGR5 and GATA6 in a normal mucosa-adenoma-adenocarcinoma sequence. (A) Immunolabeling patterns and (B) quantitative analysis of β-catenin; (C) immunolabeling patterns and (D) quantitative analysis of LGR5; and (E) immunolabeling and (F) quantitative analysis of GATA6 in representative colorectal tissues, including normal mucosa, adenomas and adenocarcinomas obtained from the same patient. Scale bar=50 µm. *P<0.05, **P<0.01. LGR5, leucine-rich repeat-containing G protein-coupled receptor 5; GATA6, GATA-binding factor 6.
See this image and copyright information in PMC

References

    1. Global Burden of Disease Cancer Collaboration. Fitzmaurice C, Dicker D, Pain A, Hamavid H, Moradi-Lakeh M, MacIntyre MF, Allen C, Hansen G, Woodbrook R, et al. The Global Burden of Cancer, 2013. JAMA Oncol. 2015;1:505–527. doi: 10.1001/jamaoncol.2015.0735. - DOI - PMC - PubMed
    1. Siegel RL, Miller KD, Fedewa SA, Ahnen DJ, Meester RGS, Barzi A, Jemal A. Colorectal cancer statistics, 2017. CA Cancer J Clin. 2017;67:177–193. doi: 10.3322/caac.21387. - DOI - PubMed
    1. Global Burden of Disease Cancer Collaboration. Fitzmaurice C, Allen C, Barber RM, Barregard L, Bhutta ZA, Brenner H, Dicker DJ, Chimed-Orchir O, Dandona R, et al. Global, regional and national cancer incidence, mortality, years of life lost, years lived with disability and disability-adjusted life-years for 32 cancer groups, 1990 to 2015: A systematic analysis for the global burden of disease study. JAMA Oncol. 2017;3:524–548. doi: 10.1001/jamaoncol.2016.5688. - DOI - PMC - PubMed
    1. Kinzler KW, Vogelstein B. Lessons from hereditary colorectal cancer. Cell. 1996;87:159–170. doi: 10.1016/S0092-8674(00)81333-1. - DOI - PubMed
    1. Fearon ER, Vogelstein B. A genetic model for colorectal tumorigenesis. Cell. 1990;61:759–767. doi: 10.1016/0092-8674(90)90186-I. - DOI - PubMed