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. 1986 Oct;166(2):553-7.
doi: 10.1016/0014-4827(86)90500-8.

Prosomes exist in plant cells too

A Kremp, M Schliephacke, U Kull, H P Schmid
Free article

Prosomes exist in plant cells too

A Kremp et al. Exp Cell Res. 1986 Oct.
Free article

Abstract

A 19S particle was purified from tobacco (Nicotiana rustica) leaf cells. Its density was determined as 1296 g/cm3 in Cs2SO4-DMSO gradients, indicating the presence of RNA and protein. Polyacrylamide gel electrophoresis (PAGE) revealed eight distinct proteins in the range of 20-30 kD and RNA in the range of 70-80 nucleotides. Electron microscopic examination showed the same raspberry-shaped structure with a central depression as described for prosomes. We conclude that tobacco 19S particles represent small cytoplasmic complexes, possessing biochemical and structural characteristics similar to the hither-to known prosomes of animal cells.

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