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. 2018 Jun;48(6):1074-1077.
doi: 10.1002/eji.201747358. Epub 2018 Mar 1.

Fast and efficient genetic engineering of hematopoietic precursor cells for the study of dendritic cell migration

Alexander Leithner  1 , Joerg Renkawitz  1 , Ingrid De Vries  1 , Robert Hauschild  1 , Hans Häcker  2 , Michael Sixt  1
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Free article

Fast and efficient genetic engineering of hematopoietic precursor cells for the study of dendritic cell migration

Alexander Leithner et al. Eur J Immunol. 2018 Jun.
Free article

Abstract

Estrogen inducible Hoxb8 leads to conditional immortalization of hematopoietic precursors. These cells can be cultured and infected with the CRISPR/Cas9 system for genome editing, circumventing resource consuming generation of mouse models. The resultant cells retain their ability to differentiate into migratory dendritic cells.

Keywords: CCL19; CCR7; CRISPR/Cas9; Cell migration; Genome editing.

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