Buffers
Cold Spring Harb Protoc.
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Abstract
Biological reactions work well only within a narrow concentration range of hydrogen ions. Paradoxically, however, many of these reactions themselves generate or consume protons. Buffers are substances that undergo reversible protonation within a particular pH range and therefore maintain the concentration of hydrogen ions within acceptable limits. This introduction describes standard buffers used in molecular cloning, including Tris buffers, Good buffers, and phosphate buffers.
© 2018 Cold Spring Harbor Laboratory Press.
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