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Comment
. 2018 Mar 5;217(3):809-811.
doi: 10.1083/jcb.201802025. Epub 2018 Feb 14.

Open-source recombinant monoclonal secondary nanobodies

Helge Ewers  1
Affiliations
Comment

Open-source recombinant monoclonal secondary nanobodies

Helge Ewers. J Cell Biol. .

Abstract

Secondary antibodies are everyday reagents in biomedical research that are generated in animals. In this issue, Pleiner et al. (2018. J. Cell Biol. https://doi.org/10.1083/jcb.201709115) describe several single domain antibody fragments against antibodies from mouse and rabbit, so-called nanobodies that are easily produced recombinantly, and characterize their use in Western blotting, enzyme-linked immunosorbent assay, and immunofluorescence assays.

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Figures

Figure 1.
Figure 1.
Nanobodies as secondary antibodies. (a) Structure of the variable domain of an alpaca heavy chain antibody (VHH). The variable loops are emphasized in yellow (PDB accession number 3OGO). (b) Structure of an IgG antibody with main nanobodies binding to Fab or Fc domains to rabbit or mouse antibodies described in the manuscript. Inset shows comparative size of nanobody (PDB accession number 1IGT).
See this image and copyright information in PMC

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References

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