Different T cell requirements for specific memory induction in normal and xid B cells

Abstract

The immune response to TTGG-A--L, a defined-sequence, branched-chain polypeptide, is regulated by MHC-linked Ir genes. TTGG-A--L specific B cells can be demonstrated in both normal and immune-defective low responder strains by activation to specific antibody secretion after immunization with TTGGAA-F gamma G, a conjugate of the hexapeptide TTGGAA and the immunogenic carrier fowl gamma-globulin. It is shown that immunization with TTGG-A--L induces specific memory B cells with equal efficiency in normal low and high responder strains but not in immune-defective low responder strains. We conclude that memory induction in xid B cells in contrast to normal B cells is dependent on MHC-restricted, carrier-specific helper T cells. Other observations also suggest a more stringent requirement for MHC-restricted, carrier-specific helper T cells in the induction of TTGGAA-specific antibody secretion by xid as compared to normal B cells. Both normal and immune-defective H-2k/b hybrids between the mutant CBA/N strain and TTGG-A--L high responder BALB.B are responders to TTGG-A--L. In contrast, normal but not immune-defective H-2k/d hybrids with responder BALB/c are responders to TTGG-A--L. This identifies H-2d as a TTGG-A--L high responder haplotype for normal B cells but a low responder haplotype for xid B cells, whereas H-2b is a high responder haplotype for both normal and xid B cells. This must reflect a quantitative or qualitative difference in Ir gene-mediated cellular interactions required for induction of antibody secretion in normal and xid B cells.