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. 2018 Feb;31(1):21-26.
Epub 2017 Feb 16.

[Prevalence and phylogenetic analysis of Chlamydia trachomatis in a population of women in Posadas, Misiones]

[Article in Spanish]
G B Jordá  1 , S E Hanke, J M Ramos-Rincón, J Mosmann, M L Lopéz, A C Entrocassi, C Cuffini
Affiliations

[Prevalence and phylogenetic analysis of Chlamydia trachomatis in a population of women in Posadas, Misiones]

[Article in Spanish]
G B Jordá et al. Rev Esp Quimioter. 2018 Feb.

Abstract
in English, Spanish

Objective: Chlamydia trachomatis is the most prevalent bacteria causing sexually transmitted infections. In women, this infection can cause cervicitis and urethritis, although it's usually asymptomatic. The aim of this study was to investigate the prevalence of C. trachomatis in women attending the lab Instituto de Previsión Social and detect the genotypes.

Methods: Endocervical samples from 505 symptomatic and asymptomatic women were assayed. It was determined the presence of C. trachomatis by PCR through amplification of a fragment of the cryptic plasmid. Positive samples were genotyped by the partial amplification of the ompA gene and analyzed phylogenetically.

Results: Forty-three positive samples were detected to infection with C. trachomatis, obtaining a prevalence of 8.5% (IC 95%: 6.4-11.3%). The prevalence of C. trachomatis was higher in women with vaginal symptoms [11.3% (30/265) vs. 5.4% (13/240)] (p = 0.018), as well as in women under 26 year-old [11.5% (28/244) vs. 6.2% (15/246)] (p = 0.021). Based on phylogenetic analysis, it was observed that 62% of the samples were genotype E, 15% genotype J, 15% genotype D, and 8% genotype F.

Conclusions: This work is the first contribution on the molecular epidemiology of C. trachomatis in the Misiones province, Argentina, which shows the rate of prevalence of this bacterium and offers information on circulating genotypes.

Introducción: Chlamydia trachomatis es una de las bacterias más prevalentes en infecciones sexualmente transmisibles. En mujeres, la infección por C. trachomatis puede causar cervicitis y uretritis, aunque suele cursar en forma asintomática. El objetivo de este trabajo fue establecer la prevalencia de C. trachomatis y detectar los genotipos circulantes en mujeres que concurren al laboratorio del Instituto de Previsión Social.

Material y métodos: Se analizaron 505 muestras endocervicales de mujeres sintomáticas y asintomáticas. Se determinó la presencia de C. trachomatis por PCR, mediante la amplificación de un fragmento del plásmido críptico. Las muestras positivas fueron genotipificadas mediante la amplificación parcial del gen ompA y se analizaron filogenéticamente.

Resultados: Se detectaron 43 muestras positivas a la infección por C. trachomatis, obteniéndose una prevalencia de 8,5% (IC 95%: 6,4-11,3%). La prevalencia de C. trachomatis fue superior en las mujeres con sintomatología vaginal [11,3% (30/265) vs. 5,4% (13/240)] (p=0,018), así como en las mujeres menores de 26 años [11,5% (28/244) vs. 6,1% (15/246)] (p=0,021). A partir del análisis filogenético, se observó que el 62% de las muestras positivas para el gen ompA pertenecieron al genotipo E, 15% al genotipo J, 15% al genotipo D y 8% al F.

Conclusiones: Este trabajo es el primer aporte sobre la epidemiología molecular de C. trachomatis en la provincia de Misiones, Argentina, que muestra la tasa de prevalencia de esta bacteria y ofrece información de genotipos circulantes.

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Conflict of interest statement

Los autores declaran no tener ningún conflicto de intereses.

Figures

Figura 1
Figura 1
Análisis Filogenético de una región del gen ompA de C. trachomatis (576 pb), construido por Maximum Likelihood usando TPM1uf+G como modelo. Los bootstraps están indicados en los nodos externos.
See this image and copyright information in PMC

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