Effects of patterned peripheral nerve stimulation on soleus spinal motor neuron excitability

Abstract

Spinal plasticity is thought to contribute to sensorimotor recovery of limb function in several neurological disorders and can be experimentally induced in animals and humans using different stimulation protocols. In healthy individuals, electrical continuous Theta Burst Stimulation (TBS) of the median nerve has been shown to change spinal motoneuron excitability in the cervical spinal cord as indexed by a change in mean H-reflex amplitude in the flexor carpi radialis muscle. It is unknown whether continuous TBS of a peripheral nerve can also shift motoneuron excitability in the lower limb. In 26 healthy subjects, we examined the effects of electrical TBS given to the tibial nerve in the popliteal fossa on the excitability of lumbar spinal motoneurons as measured by H-reflex amplitude of the soleus muscle evoked by tibial nerve stimulation. Continuous TBS was given at 110% of H-reflex threshold intensity and compared to non-patterned regular electrical stimulation at 15 Hz. To disclose any pain-induced effects, we also tested the effects of TBS at individual sensory threshold. Moreover, in a subgroup of subjects we evaluated paired-pulse inhibition of H-reflex. Continuous TBS at 110% of H-reflex threshold intensity induced a short-term reduction of H-reflex amplitude. The other stimulation conditions produced no after effects. Paired-pulse H-reflex inhibition was not modulated by continuous TBS or non-patterned repetitive stimulation at 15 Hz. An effect of pain on the results obtained was discarded, since non-patterned 15 Hz stimulation at 110% HT led to pain scores similar to those induced by EcTBS at 110% HT, but was not able to induce any modulation of the H reflex amplitude. Together, the results provide first time evidence that peripheral continuous TBS induces a short-lasting change in the excitability of spinal motoneurons in lower limb circuitries. Future studies need to investigate how the TBS protocol can be optimized to produce a larger and longer effect on spinal cord physiology and whether this might be a useful intervention in patients with excessive excitability of the spinal motorneurons.

Fig 1. Experimental set-up.

A) Schematic view of the monosynaptic reflex and H reflex recordings. B) Theta burst and non-patterned 15Hz interventions. C) The experimental protocol.

Fig 2. EcTBS_110HT main effects.

A) Time course of the H/Mmax ratio normalized to the baseline. Values are mean and error bars are standard error of the mean. B) Effects of EcTBS_110HT intervention on the H/Mmax ratio in individual subjects (Post 1/Baseline). C) Vas scores in the experiments at above HR thresholds. Vas scores in the experiment at above HR thresholds. Values are mean and error bars are standard deviations.

Fig 3. EcTBS_110HT effects in a representative subject.

A) H reflex in a representative subject before and after EcTBS110HT intervention. B) Paired pulse study (H2/H1) in a representative subject before and after EcTBS110HT intervention.