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. 2018:1748:157-171.
doi: 10.1007/978-1-4939-7698-0_12.

Assessing Sertoli Cell Metabolic Activity

Ivana Jarak  1   2 , Pedro F Oliveira  3   4   5   6 , Gustavo Rindone  7 , Rui A Carvalho  2 , María N Galardo  7 , María F Riera  7 , Silvina B Meroni  7 , Marco G Alves  8
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Assessing Sertoli Cell Metabolic Activity

Ivana Jarak et al. Methods Mol Biol. 2018.

Abstract

Nuclear magnetic resonance (NMR)-based metabolomics is widely used in the research of metabolic conditions of complex biological systems under various conditions, and its use has been found in the field of male fertility. Here we describe the implementation of total and targeted NMR-based metabolomics in the research on Sertoli cell metabolism. Main principles and techniques of cell medium, cellular extracts, and intact cells are explained, as well as some classical experiments that can give complementary information on the Sertoli cell metabolism.

Keywords: Extracellular; Fertility; Glucose uptake; Intracellular; Lipids; Metabolism; Metabolomics; NMR; Sertoli.

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