Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Feb;21(2):212-218.
doi: 10.22038/IJBMS.2018.24693.6136.

Comparison of the efficacy of piascledine and transforming growth factor β1 on chondrogenic differentiation of human adipose-derived stem cells in fibrin and fibrin-alginate scaffolds

Batool Hashemibeni  1 , Ali Valiani  1 , Mojtaba Esmaeli  1 , Mohammad Kazemi  2 , Maryam Aliakbari  1 , Farhad Golshan Iranpour  1
Affiliations

Comparison of the efficacy of piascledine and transforming growth factor β1 on chondrogenic differentiation of human adipose-derived stem cells in fibrin and fibrin-alginate scaffolds

Batool Hashemibeni et al. Iran J Basic Med Sci. 2018 Feb.

Abstract

Objectives: The aim of this study was to compare the chondrogenic induction potential of Piascledine and TGF-β1 on adipose-derived stem cells (ADSCs) in fibrin and fibrin-alginate scaffolds.

Materials and methods: Human subcutaneous adipose tissues were harvested from three patients who were scheduled to undergo liposuction. Isolated ADSCs were proliferated in a culture medium. Then, the cells were seeded in fibrin or fibrin-alginate scaffolds and cultured for 14 days in a chondrogenic medium containing Piascledine, TGF-β1, or both. The rate of cell proliferation and survival was evaluated by using MTT [3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide] assay and the rate of the expression of type II collagen, aggrecan, and type X collagen genes was evaluated by real-time polymerase chain reaction (real-time PCR) method.

Results: The MTT results showed that Piascledine is able to enhance the proliferation and survival of ADSCs in fibrin scaffolds in comparison to other groups (P<0.05). Real-time PCR evaluation revealed that the expression of type II collagen was higher in TGF- β1groups, but the expression of aggrecan was higher in TGF-β1 alone or along with Piascledine in fibrin-alginate scaffolds. Furthermore, the expression of type X collagen was lower in Piascledine alone or along with TGF-β1 in fibrin scaffold.

Conclusion: Piascledine can enhance the proliferation and differentiation of ADSCs in fibrin scaffolds.

Keywords: Chondrogenesis; Piascledine; Stem cells; Tissue engineering; Transforming growth-factor beta 1.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Inverted microscope images of the monolayer culture of ADSCs in the third passage (magnification x 40) (a) on the 2nd day (b) on the 6th day
Figure 2
Figure 2
Photographs of chondrocytes seeded in fibrin (a) and fibrin-alginate (b) scaffolds
Figure 3
Figure 3
MTT assay results 14 days after the culture of ADSCs in chondrogenic medium supplemented with TGF-β1, Piascledine or both in fibrin or fibrin-alginate scaffolds. Asterisks indicate significant differences between groups. *P<0.05, ** P<0.01, *** P<0.001
Figure 4
Figure 4
The results of collagen II (a), collagen Χ (b) and aggrecan genes expression in different groups 14 days after the culture of adipose-derived stem cells. Data are presented as mean ± SD. Error bars represent the standard deviation of the mean. Asterisks indicate significant differences between groups. N.S: Not Significant, *P<0.05, ** P<0.01, *** P<0.001. Relative quantity (RQ) indicates the relative level of gene expression
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Hardingham T, Tew S, Murdoch A. Tissue engineering: Chondrocytes and cartilage. Arthritis Res. 2002;4:63–68. - PMC - PubMed
    1. Mitchell N, Shepard N. The resurfacing of adult rabbit articular cartilage by multiple perforations through the subchondral bone. J Bone Joint Surg Am. 1976;58:230–233. - PubMed
    1. Mardani M, Hashemibeni B, Ansar MM, Esfahani SH, Kazemi M, Goharian V, et al. Comparison between chondrogenic markers of differentiated chondrocytes from adipose derived stem cells and articular chondrocytes in vitro. Iran J Basic Med Sci. 2013;16:763–773. - PMC - PubMed
    1. Colter DC, Sekiya I, Prockop DJ. Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells. Proc Natl Acad Sci USA. 2001;98:7841–7845. - PMC - PubMed
    1. Hahemibeni B, Razavi Sh, Esfandiary E, Karbasi S, Mardani M, Nasresfahani M. Induction of chondrogenic differentiation of human adipose-derived stem cells with TGF-β3 in pellet culture system. Iran J Basic Med Sci. 2008;11:10–17.

LinkOut - more resources