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. 2018 Feb;21(2):212-218.
doi: 10.22038/IJBMS.2018.24693.6136.

Comparison of the efficacy of piascledine and transforming growth factor β1 on chondrogenic differentiation of human adipose-derived stem cells in fibrin and fibrin-alginate scaffolds

Batool Hashemibeni  1 , Ali Valiani  1 , Mojtaba Esmaeli  1 , Mohammad Kazemi  2 , Maryam Aliakbari  1 , Farhad Golshan Iranpour  1
Affiliations

Comparison of the efficacy of piascledine and transforming growth factor β1 on chondrogenic differentiation of human adipose-derived stem cells in fibrin and fibrin-alginate scaffolds

Batool Hashemibeni et al. Iran J Basic Med Sci. 2018 Feb.

Abstract

Objectives: The aim of this study was to compare the chondrogenic induction potential of Piascledine and TGF-β1 on adipose-derived stem cells (ADSCs) in fibrin and fibrin-alginate scaffolds.

Materials and methods: Human subcutaneous adipose tissues were harvested from three patients who were scheduled to undergo liposuction. Isolated ADSCs were proliferated in a culture medium. Then, the cells were seeded in fibrin or fibrin-alginate scaffolds and cultured for 14 days in a chondrogenic medium containing Piascledine, TGF-β1, or both. The rate of cell proliferation and survival was evaluated by using MTT [3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide] assay and the rate of the expression of type II collagen, aggrecan, and type X collagen genes was evaluated by real-time polymerase chain reaction (real-time PCR) method.

Results: The MTT results showed that Piascledine is able to enhance the proliferation and survival of ADSCs in fibrin scaffolds in comparison to other groups (P<0.05). Real-time PCR evaluation revealed that the expression of type II collagen was higher in TGF- β1groups, but the expression of aggrecan was higher in TGF-β1 alone or along with Piascledine in fibrin-alginate scaffolds. Furthermore, the expression of type X collagen was lower in Piascledine alone or along with TGF-β1 in fibrin scaffold.

Conclusion: Piascledine can enhance the proliferation and differentiation of ADSCs in fibrin scaffolds.

Keywords: Chondrogenesis; Piascledine; Stem cells; Tissue engineering; Transforming growth-factor beta 1.

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Figures

Figure 1
Figure 1
Inverted microscope images of the monolayer culture of ADSCs in the third passage (magnification x 40) (a) on the 2nd day (b) on the 6th day
Figure 2
Figure 2
Photographs of chondrocytes seeded in fibrin (a) and fibrin-alginate (b) scaffolds
Figure 3
Figure 3
MTT assay results 14 days after the culture of ADSCs in chondrogenic medium supplemented with TGF-β1, Piascledine or both in fibrin or fibrin-alginate scaffolds. Asterisks indicate significant differences between groups. *P<0.05, ** P<0.01, *** P<0.001
Figure 4
Figure 4
The results of collagen II (a), collagen Χ (b) and aggrecan genes expression in different groups 14 days after the culture of adipose-derived stem cells. Data are presented as mean ± SD. Error bars represent the standard deviation of the mean. Asterisks indicate significant differences between groups. N.S: Not Significant, *P<0.05, ** P<0.01, *** P<0.001. Relative quantity (RQ) indicates the relative level of gene expression
See this image and copyright information in PMC

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