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. 2018 Apr:63:44-50.
doi: 10.1016/j.ejop.2018.01.007. Epub 2018 Feb 6.

A novel TaqMan® assay for Nosema ceranae quantification in honey bee, based on the protein coding gene Hsp70

Giovanni Cilia  1 , Riccardo Cabbri  2 , Giacomo Maiorana  3 , Ilaria Cardaio  4 , Raffaele Dall'Olio  4 , Antonio Nanetti  4
Affiliations

A novel TaqMan® assay for Nosema ceranae quantification in honey bee, based on the protein coding gene Hsp70

Giovanni Cilia et al. Eur J Protistol. 2018 Apr.

Abstract

Nosema ceranae is now a widespread honey bee pathogen with high incidence in apiculture. Rapid and reliable detection and quantification methods are a matter of concern for research community, nowadays mainly relying on the use of biomolecular techniques such as PCR, RT-PCR or HRMA. The aim of this technical paper is to provide a new qPCR assay, based on the highly-conserved protein coding gene Hsp70, to detect and quantify the microsporidian Nosema ceranae affecting the western honey bee Apis mellifera. The validation steps to assess efficiency, sensitivity, specificity and robustness of the assay are described also.

Keywords: Apis mellifera; Hsp70 gene; Nosema ceranae; Nosemosis; TaqMan(®) assay; qPCR.

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