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. 2018 Apr 18;80(4):590-593.
doi: 10.1292/jvms.17-0581. Epub 2018 Feb 20.

Isolation and molecular characterization of parainfluenza virus 5 in diarrhea-affected piglets in China

Ning Jiang  1 , Enyu Wang  1 , Donghua Guo  1 , Xin Wang  1 , Mingjun Su  1 , Fanzhi Kong  1 , Dongwei Yuan  1 , Junjun Zhai  1 , Dongbo Sun  1
Affiliations

Isolation and molecular characterization of parainfluenza virus 5 in diarrhea-affected piglets in China

Ning Jiang et al. J Vet Med Sci. .

Abstract

Recently, parainfluenza virus 5 (PIV5) infection has been increasingly reported in mammals. In this study, five PIV5 strains were isolated from diarrhea-affected piglets from four provinces or municipalities in China. An F-gene-based phylogenetic tree indicated that the five isolated strains were closely related to the PIV5 strain ZJQ-221 from a lesser panda in China, and the PIV5 strain 1168-1 from a dog in South Korea. The new isolates differed genetically from other pig, calf, rhesus macaque kidney cells, human, and dog PIV5 reference strains. Our study reveals the presence of PIV5 in intestinal tissue samples collected from diarrhea-affected piglets, and provides novel information regarding the epidemiology and tissue tropism of PIV5.

Keywords: diarrhea-affected piglet; isolation; parainfluenza virus 5.

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Figures

Fig. 1.
Fig. 1.
Virus-specific CPE of the five PIV5 strains on Vero E6 cells at a magnification of 200 × (fifth passage, 36 hr post infections). A, Mock-infected Vero E6 cells; B, PIV5-infected Vero E6 cells with strain HLJ2015/DP1-1/PIV5; C, PIV5-infected Vero E6 cells with strain HLJ2015/DP2-1/PIV5; D, PIV5-infected Vero E6 cells with strain SH/2015/122/PIV5; E, PIV5-infected Vero E6 cells with strain HuB/YC/2015/PIV5; F, PIV5-infected Vero E6 cells with strain JX/2015/1221/PIV5.
Fig. 2.
Fig. 2.
Transmission electron microcopy of the supernatants of the virus infected Vero E6 cells by using negative staining with phosphotungstic acid. The micrograph is representative of the five PIV5 strains identified in our study and demonstrates the PIV5 ultrastructure. (Scale bar=200 nm)
Fig. 3.
Fig. 3.
The electrophoretic diagram of RT-PCR amplification of the F gene from the five newly isolated PIV5 strains on 0.8% agarose gel electrophoresis with ethidium bromide staining. Lane M, DNA Marker DL2000; Lane 1, negative control; Lane 2, strain HLJ2015/DP1-1/PIV5; Lane 3, strain HLJ2015/DP2-1/PIV5; Lane 4, strain SH/2015/122/PIV5; Lane 5, strain HuB/YC/2015/PIV5; Lane 6, strain JX/2015/1221/PIV5.
Fig. 4.
Fig. 4.
F-gene based phylogenetic tree of the five PIV5 strains using selected representative PIV5 strains as references. The red circles indicate the PIV5 strains identified in our study.
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