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. 2018 Feb 20;23(2):463.
doi: 10.3390/molecules23020463.

Qualitative and Quantitative Phytochemical Analysis of Different Extracts from Thymus algeriensis Aerial Parts

Nassima Boutaoui  1 , Lahcene Zaiter  2 , Fadila Benayache  3 , Samir Benayache  4 , Simone Carradori  5 , Stefania Cesa  6 , Anna Maria Giusti  7 , Cristina Campestre  8 , Luigi Menghini  9 , Denise Innosa  10 , Marcello Locatelli  11
Affiliations

Qualitative and Quantitative Phytochemical Analysis of Different Extracts from Thymus algeriensis Aerial Parts

Nassima Boutaoui et al. Molecules. .

Abstract

This study was performed to evaluate the metabolite recovery from different extraction methods applied to Thymusalgeriensis aerial parts. A high-performance liquid chromatographic method using photodiode array detector with gradient elution has been developed and validated for the simultaneous estimation of different phenolic compounds in the extracts and in their corresponding purified fractions. The experimental results show that microwave-assisted aqueous extraction for 15 min at 100 °C gave the most phenolics-enriched extract, reducing extraction time without degradation effects on bioactives. Sixteen compounds were identified in this extract, 11 phenolic compounds and five flavonoids, all known for their biological activities. Color analysis and determination of chlorophylls and carotenoids implemented the knowledge of the chemical profile of this plant.

Keywords: HPLC-PDA; MAE; SFE; Thymus algeriensis; color analysis; pigments.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Schematic flowchart performed on Thymus algeriensis aerial parts.
Figure 2
Figure 2
Reflectance analysis of Thymus algeriensis aerial parts.
Figure 3
Figure 3
Pigments concentration in T. algeriensis aerial parts (µg/mg ± SD).
See this image and copyright information in PMC

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