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. 2018 Apr 26;62(5):e02240-17.
doi: 10.1128/AAC.02240-17. Print 2018 May.

Detection of TR34/L98H CYP51A Mutation through Passive Surveillance for Azole-Resistant Aspergillus fumigatus in the United States from 2015 to 2017

Elizabeth L Berkow  1 , Natalie S Nunnally  1 , Alex Bandea  1 , Randall Kuykendall  1 , Karlyn Beer  1 , Shawn R Lockhart  2
Affiliations

Detection of TR34/L98H CYP51A Mutation through Passive Surveillance for Azole-Resistant Aspergillus fumigatus in the United States from 2015 to 2017

Elizabeth L Berkow et al. Antimicrob Agents Chemother. .

Abstract

The emergence of azole-resistant Aspergillus fumigatus has become a clinical problem in many parts of the world. Several amino acid mutations in the azole target protein Cyp51Ap contribute to this resistance, with the most concerning being the environmentally derived TR34/L98H and TR46/Y121F/T289A mutations. Here, we performed passive surveillance to assess a sample of the A. fumigatus population in the United States for the presence of these mutations. We found 1.4% of those isolates to exhibit elevated MIC via broth microdilution, and five of those isolates harbored the TR34/L98H mutation.

Keywords: Aspergillus fumigatus; CYP51A mutation; TR34/L98H; TR46/Y121F/T289A; azole resistance.

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Figures

FIG 1
FIG 1
States of origin of 1,356 A. fumigatus isolates collected through passive surveillance, 2015 to 2017.
FIG 2
FIG 2
Susceptibility profiles for A. fumigatus isolates via Etest method. Dashed line represents the CLSI microdilution method ECV cutoff between wild type and non-wild type.
See this image and copyright information in PMC

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