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Published Erratum

Correction: Enzymatic lipid oxidation by eosinophils propagates coagulation, hemostasis, and thrombotic disease

Stefan Uderhardt et al. J Exp Med. .
No abstract available

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Figures

Figure 4.
Figure 4.
Ca2+-dependent exposure of aminophospholipids by eosinophils promote thrombin generation. (A) Flow cytometry analysis of the binding of annexin V (AxV) to aminophospholipids on the surface of resting or ADP-stimulated mouse eosinophils. Histograms show representative annexin V stainings, and bar graphs show mean geometric fluorescence intensities (gMFI). (B) LC/MS/MS-based quantification of the exposure of the aminophospholipids PE and PS in mouse eosinophils in response to ADP stimulation. (C, left) Calibrated thrombin generation assays with resting or ADP-stimulated mouse eosinophils in the presence of annexin V. (Right) Bar graphs show endogenous thrombin potential (ETP; nM*min) and peak of thrombin generation (peak; nM). (D) Flow cytometry analysis of annexin V binding on mouse eosinophils over time in the presence of calcium ionophore A23187, ADP, or vehicle. (E) Flow cytometry analysis of annexin V binding on mouse eosinophils in the presence of tannic acid (TA) or intracellular Ca2+-chelator BAP​TA/AM. Bar graphs show geometric mean fluorescence intensity. (F) Flow cytometry–based analysis of intracellular Ca2+ signaling, indicated by Fluo3/FuraRed ratio, over time in a Ca2+-free environment. Where indicated (arrow and Ca2+), CaCl2 at a final concentration of 1 mM was added. (G) Flow cytometry–based analysis of intracellular Ca2+ signaling, indicated by Fluo3/FuraRed ratio, over time in a Ca2+-free environment. (H) Postulated mechanism of a sequential generation and Ca2+-dependent externalization of aminophospholipids (APL) at the surface of eosinophils. OH indicates hydroxyl group. Data are representative of at least three independent experiments. Error bars represent SEM. *, P < 0.05; ***, P < 0.001.
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Erratum for

  • Enzymatic lipid oxidation by eosinophils propagates coagulation, hemostasis, and thrombotic disease.
    Uderhardt S, Ackermann JA, Fillep T, Hammond VJ, Willeit J, Santer P, Mayr M, Biburger M, Miller M, Zellner KR, Stark K, Zarbock A, Rossaint J, Schubert I, Mielenz D, Dietel B, Raaz-Schrauder D, Ay C, Gremmel T, Thaler J, Heim C, Herrmann M, Collins PW, Schabbauer G, Mackman N, Voehringer D, Nadler JL, Lee JJ, Massberg S, Rauh M, Kiechl S, Schett G, O'Donnell VB, Krönke G. Uderhardt S, et al. J Exp Med. 2017 Jul 3;214(7):2121-2138. doi: 10.1084/jem.20161070. Epub 2017 May 31. J Exp Med. 2017. PMID: 28566277 Free PMC article.

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