Redirection of neuroblast migration from the rostral migratory stream into a lesion in the prefrontal cortex of adult rats

Abstract

Clinical treatment of structural brain damage today is largely limited to symptomatic approaches and the avoidance of secondary injury. However, neuronal precursor cells are constantly produced within specified regions of the mammalian brain throughout life. Here we evaluate the potential of the known chemoattractive properties of the glycoprotein laminin on neuroblasts to relocate the cells into damaged brain areas. Injection of a thin laminin tract, leading from the rostral migratory stream to an excitotoxic lesion within the medial prefrontal cortex of rats, enabled neuroblasts to migrate away from their physiological route towards the olfactory bulb into the lesion site. Once they reached the damaged tissue, they migrated further in a non-uniform orientation within the lesion. Furthermore, our data indicate that the process of diverted migration is still active 6 weeks after the treatment and that at least some of the neuroblasts are capable of maturing into adult neurons.

Keywords: Brain lesion; Laminin; Migration cue; Neuroblast migration; Rostral migratory stream; Structural recovery.