Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Feb;25(2):339-348.
doi: 10.1016/j.sjbs.2017.03.020. Epub 2017 May 5.

Impact of pH and butyric acid on butanol production during batch fermentation using a new local isolate of Clostridium acetobutylicum YM1

Najeeb Kaid Nasser Al-Shorgani  1   2 , Mohd Sahaid Kalil  1 , Wan Mohtar Wan Yusoff  3 , Aidil Abdul Hamid  3
Affiliations

Impact of pH and butyric acid on butanol production during batch fermentation using a new local isolate of Clostridium acetobutylicum YM1

Najeeb Kaid Nasser Al-Shorgani et al. Saudi J Biol Sci. 2018 Feb.

Abstract

The effect of pH and butyric acid supplementation on the production of butanol by a new local isolate of Clostridium acetobutylicum YM1 during batch culture fermentation was investigated. The results showed that pH had a significant effect on bacterial growth and butanol yield and productivity. The optimal initial pH that maximized butanol production was pH 6.0 ± 0.2. Controlled pH was found to be unsuitable for butanol production in strain YM1, while the uncontrolled pH condition with an initial pH of 6.0 ± 0.2 was suitable for bacterial growth, butanol yield and productivity. The maximum butanol concentration of 13.5 ± 1.42 g/L was obtained from cultures grown under the uncontrolled pH condition, resulting in a butanol yield (YP/S ) and productivity of 0.27 g/g and 0.188 g/L h, respectively. Supplementation of the pH-controlled cultures with 4.0 g/L butyric acid did not improve butanol production; however, supplementation of the uncontrolled pH cultures resulted in high butanol concentrations, yield and productivity (16.50 ± 0.8 g/L, 0.345 g/g and 0.163 g/L h, respectively). pH influenced the activity of NADH-dependent butanol dehydrogenase, with the highest activity obtained under the uncontrolled pH condition. This study revealed that pH is a very important factor in butanol fermentation by C. acetobutylicum YM1.

Keywords: ABE, acetone-butanol-ethanol; Butanol; Butyric acid; Clostridium acetobutylicum YM1; NADH-BDH, NADH-dependent butanol dehydrogenase; NADH-dependent butanol dehydrogenase; pH.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Effect of initial pH on butanol production by C. acetobutylicum YM1.
Fig. 2
Fig. 2
Effect of uncontrolled pH (initial pH 6.0) on cell growth, pH, glucose utilization and the production of butanol and butyric acid.
Fig. 3
Fig. 3
Effect of controlled pH 6.0 on cell growth, pH, glucose utilization and the production of butanol and butyric acid.
Fig. 4
Fig. 4
Effect of controlled pH at 5.5 on cell growth, pH, glucose utilization and the production of butanol and butyric acid.
Fig. 5
Fig. 5
Effect of controlled pH at 4.8 on cell growth, pH, glucose utilization and the production of butanol and butyric acid.
Fig. 6
Fig. 6
Profile of cell growth, pH, butyric acid, glucose consumption and butanol production under two-stage pH control: the pH was controlled at 6.0 for 12 h, and then the fermentation was run without control.
Fig. 7
Fig. 7
Profile of cell growth, pH, butyric acid, glucose consumption and butanol production under two-stage pH control: the culture was started with an initial pH of 6.0 and run without control the pH. After 12 h it was controlled at 6.0 for the remainder of the fermentation time.
Fig. 8
Fig. 8
Profile of cell growth, butyric acid, glucose consumption and butanol production in batch culture of YM1 under uncontrolled pH condition and a 4 g/L butyric acid supplementation.
Fig. 9
Fig. 9
Profile of cell growth, butyric acid, glucose consumption and butanol production in a batch culture of YM1 under controlled pH at 6.0 with initial supplementation with 4 g/L butyric acid.
Fig. 10
Fig. 10
Profile of cell growth, butyric acid, glucose consumption and butanol production in a batch culture of YM1 under controlled pH conditions (pH 6.0) with supplementation of 4 g/L butyric acid after 14 h of fermentation.
Fig. 11
Fig. 11
Profile of growth, butanol production and NADH-dependent butanol dehydrogenase activity in butanol fermentation by C. acetobutylicum YM1 (a) in non-controlled pH culture, (b) controlled pH fermentation at 6.0, (c) fermentation controlled at pH 6.0 for 12 h and then allowed to proceed without control and (d) comparison of the specific activity of NADH-BDH under three different pH strategies.
See this image and copyright information in PMC

References

    1. Abdeshahian P., Al-Shorgani N.K.N., Salih N.K.M., Shukor H., Kadier A., Hamid A.A., Kalil M.S. The production of biohydrogen by a novel strain Clostridium sp. YM1 in dark fermentation process. Int. J. Hydrogen Energy. 2014;39(24):12524–12531.
    1. Al-Shorgani N.K.N., Ali E., Kalil M.S., Yusoff W.M.W. Bioconversion of butyric acid to butanol by Clostridium saccharoperbutylacetonicum N1–4 (ATCC 13564) in a limited nutrient medium. BioEnergy Res. 2012;5(2):287–293.
    1. Al-Shorgani N.K.N., Kalil M.S., Ali E., Hamid A.A., Yusoff W.M.W. The use of pretreated palm oil mill effluent for acetone–butanol–ethanol fermentation by Clostridium saccharoperbutylacetonicum N1–4. Clean Technol. Environ. Policy. 2012;14(5):879–887.
    1. Al-Shorgani N.K.N., Kalil M.S., Yusoff W.M.W. Biobutanol production from rice bran and de-oiled rice bran by Clostridium saccharoperbutylacetonicum N1–4. Bioprocess Biosyst. Eng. 2012;35(5):817–826. - PubMed
    1. Al-Shorgani N.K.N., Kalil M.S., Yusoff W.M.W. Fermentation of sago starch to biobutanol in a batch culture using Clostridium saccharoperbutylacetonicum N1–4 (ATCC 13564) Ann. Microbiol. 2012;62(3):1059–1070.

LinkOut - more resources