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Review
. 2018 Feb 2:9:118.
doi: 10.3389/fimmu.2018.00118. eCollection 2018.

Next-Generation Sequencing of Antibody Display Repertoires

Romain Rouet  1 , Katherine J L Jackson  1 , David B Langley  1 , Daniel Christ  1   2
Affiliations
Review

Next-Generation Sequencing of Antibody Display Repertoires

Romain Rouet et al. Front Immunol. .

Abstract

In vitro selection technology has transformed the development of therapeutic monoclonal antibodies. Using methods such as phage, ribosome, and yeast display, high affinity binders can be selected from diverse repertoires. Here, we review strategies for the next-generation sequencing (NGS) of phage- and other antibody-display libraries, as well as NGS platforms and analysis tools. Moreover, we discuss recent examples relating to the use of NGS to assess library diversity, clonal enrichment, and affinity maturation.

Keywords: antibody display technology; antibody libraries; antibody therapeutics; in vitro selection; next-generation sequencing; phage display.

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