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. 2018:1745:97-112.
doi: 10.1007/978-1-4939-7680-5_6.

X-Ray Fluorescence-Detected Flow Cytometry

Andrew M Crawford  1 , James E Penner-Hahn  2
Affiliations

X-Ray Fluorescence-Detected Flow Cytometry

Andrew M Crawford et al. Methods Mol Biol. 2018.

Abstract

X-ray fluorescence-detected flow cytometry can enable the detection and characterization of ultra-trace, trace, and bulk elemental content at the cellular level using synchrotron-induced x-ray emission from fully aquated actively respiring cells. Although very much still a technique in development, this technique has been used to characterize cell-to-cell elemental variability in bovine red blood cells, Saccharomyces cerevisiae, and NIH3T3 mouse fibroblasts. Herein we describe the experimental setup and the key methodological aspects of data collection and processing.

Keywords: Cells; Cytometer; Flow cytometer; Metallome; X-ray fluorescence.

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