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. 2018 Feb 3:6:20-23.
doi: 10.7150/jgen.22986. eCollection 2018.

High Quality Draft Genomes of the Type Strains Geobacillus thermocatenulatus DSM 730T, G. uzenensis DSM 23175T And Parageobacillus galactosidasius DSM 18751T

Winnie Thabisa Ramaloko  1 , Nadine Koen  1 , Shamara Polliack  1 , Habibu Aliyu  1   2 , Pedro Humberto Lebre  1 , Teresa Mohr  2 , Florian Oswald  2 , Michaela Zwick  2 , Daniel Ray Zeigler  3 , Anke Neumann  2 , Christoph Syldatk  2 , Don Arthur Cowan  1 , Pieter De Maayer  4
Affiliations

High Quality Draft Genomes of the Type Strains Geobacillus thermocatenulatus DSM 730T, G. uzenensis DSM 23175T And Parageobacillus galactosidasius DSM 18751T

Winnie Thabisa Ramaloko et al. J Genomics. .

Abstract

The thermophilic 'Geobacilli' are important sources of thermostable enzymes and other biotechnologically relevant macromolecules. The present work reports the high quality draft genome sequences of previously unsequenced type strains of Geobacillus uzenensis (DSM 23175T), G. thermocatenulatus (DSM 730T) and Parageobacillus galactosidasius (DSM 18751T). Phylogenomic analyses revealed that DSM 18751T and DSM 23175T represent later heterotypic synonyms of P. toebii and G. subterraneus, respectively, while DSM 730T represents the type strain for the species G. thermocatenulatus. These genome sequences will contribute towards a deeper understanding of the ecological and biological diversity and the biotechnological exploitation of the 'geobacilli'.

Keywords: Firmicutes; Geobacillus; Illumina HiSeq sequencing; Parageobacillus; phylogenomics; thermophile.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interest exists.

Figures

Fig 1
Fig 1
EggNOG functional classification of proteins encoded on the three sequenced genomes. (A) Proportions (%) of proteins in each of the EggNOG super-functional categories Information processing and storage (orange), Cellular processing and signalling (yellow), Metabolism (purple) and Poorly characterized (grey). (B) Relative proportions of proteins involved in Energy metabolism (C), Amino acid transport and metabolism (E), Carbohydrate transport and metabolism (G), Lipid metabolism (I) and DNA replication, recombination and repair (L) for G. thermocatenulatus DSM 730T (blue bars), G. uzenensis DSM 23175T (maroon bars) and P. galatctosidasius DSM 18751T (green bars).
Fig 2
Fig 2
Core genome phylogeny of the three sequenced strains. A Maximum Likelihood phylogeny was constructed on the basis of 1,355 core proteins of G. uzenensis DSM 23175T, G. thermocatenulatus DSM 730T, G. uzenensis DSM 23175T and P. galatctosidasius DSM 18751T as well as 9 and 6 additional Geobacillus and Parageobacillus type strains, respectively. Anoxybacillus flavithermus DSM 2641T was used as outgroup to root the tree.
See this image and copyright information in PMC

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