Candidate genes have sex-specific effects on timing of spring migration and moult speed in a long-distance migratory bird

Abstract

The timing of major life-history events, such as migration and moult, is set by endogenous circadian and circannual clocks, that have been well characterized at the molecular level. Conversely, the genetic sources of variation in phenology and in other behavioral traits have been sparsely addressed. It has been proposed that inter-individual variability in the timing of seasonal events may arise from allelic polymorphism at phenological candidate genes involved in the signaling cascade of the endogenous clocks. In this study of a long-distance migratory passerine bird, the willow warbler Phylloscopus trochilus, we investigated whether allelic variation at 5 polymorphic loci of 4 candidate genes (Adcyap1, Clock, Creb1, and Npas2), predicted 2 major components of the annual schedule, namely timing of spring migration across the central Mediterranean sea and moult speed, the latter gauged from ptilochronological analyses of tail feathers moulted in the African winter quarters. We identified a novel Clock gene locus (Clock region 3) showing polyQ polymorphism, which was however not significantly associated with any phenotypic trait. Npas2 allele size predicted male (but not female) spring migration date, with males bearing longer alleles migrating significantly earlier than those bearing shorter alleles. Creb1 allele size significantly predicted male (but not female) moult speed, longer alleles being associated with faster moult. All other genotype-phenotype associations were statistically non-significant. These findings provide new evidence for a role of candidate genes in modulating the phenology of different circannual activities in long-distance migratory birds, and for the occurrence of sex-specific candidate gene effects.

Keywords: Adcyap1; avian migration; candidate genes; clock; phenology; ptilochronology.

Figure 1.

Migration date (1 = January 1) in relation to Npas2 mean allele size in (A) male and (B) female willow warblers. The line represents simple linear regression with a statistically significant (P < 0.05) slope. The correlation coefficient (Pearson’s r) is also shown.

Figure 2.

FGR (residuals of a regression of GBW on feather length; see Materials and Methods) versus Creb1 mean allele size in (A) male and (B) female willow warblers. High FGR values are assumed to reflect faster moult. The line represents simple linear regression with a statistically significant (P < 0.05) slope. The correlation coefficient (Pearson’s r) is also shown (the result for females was similar after removing the 2 extreme data points with FGR < −0.40; details not show for brevity).