. 2018 Mar;70(3):167-175.

doi: 10.1007/s10858-018-0167-4. Epub 2018 Feb 28.

¹³C APSY-NMR for sequential assignment of intrinsically disordered proteins

Maria Grazia Murrali¹, Marco Schiavina¹, Valerio Sainati¹, Wolfgang Bermel², Roberta Pierattelli^{3

4}, Isabella C Felli^{5

6}

Affiliations

¹ CERM, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy.
² Bruker BioSpin GmbH, Silberstreifen, 76287, Rheinstetten, Germany.
³ CERM, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy. pierattelli@cerm.unifi.it.
⁴ Department of Chemistry "Ugo Schiff", University of Florence, 50019, Sesto Fiorentino, Florence, Italy. pierattelli@cerm.unifi.it.
⁵ CERM, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy. felli@cerm.unifi.it.
⁶ Department of Chemistry "Ugo Schiff", University of Florence, 50019, Sesto Fiorentino, Florence, Italy. felli@cerm.unifi.it.

PMID: 29492731
DOI: 10.1007/s10858-018-0167-4

¹³C APSY-NMR for sequential assignment of intrinsically disordered proteins

Maria Grazia Murrali et al. J Biomol NMR. 2018 Mar.

. 2018 Mar;70(3):167-175.

doi: 10.1007/s10858-018-0167-4. Epub 2018 Feb 28.

Authors

Maria Grazia Murrali¹, Marco Schiavina¹, Valerio Sainati¹, Wolfgang Bermel², Roberta Pierattelli^{3

4}, Isabella C Felli^{5

6}

Affiliations

¹ CERM, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy.
² Bruker BioSpin GmbH, Silberstreifen, 76287, Rheinstetten, Germany.
³ CERM, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy. pierattelli@cerm.unifi.it.
⁴ Department of Chemistry "Ugo Schiff", University of Florence, 50019, Sesto Fiorentino, Florence, Italy. pierattelli@cerm.unifi.it.
⁵ CERM, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy. felli@cerm.unifi.it.
⁶ Department of Chemistry "Ugo Schiff", University of Florence, 50019, Sesto Fiorentino, Florence, Italy. felli@cerm.unifi.it.

PMID: 29492731
DOI: 10.1007/s10858-018-0167-4

Abstract

The increasingly recognized biological relevance of intrinsically disordered proteins requires a continuous expansion of the tools for their characterization via NMR spectroscopy, the only technique so far able to provide atomic-resolution information on these highly mobile macromolecules. Here we present the implementation of projection spectroscopy in ¹³C-direct detected NMR experiments to achieve the sequence specific assignment of IDPs. The approach was used to obtain the complete backbone assignment at high temperature of α-synuclein, a paradigmatic intrinsically disordered protein.

Keywords: 13C detection; Assignment; IDPs; Intrinsically disordered proteins; NUS.

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References

1. Annu Rev Biophys. 2008;37:215-46 - PubMed
1. Chem Rev. 2014 Jul 9;114(13):6844-79 - PubMed
1. Methods Enzymol. 2001;339:258-70 - PubMed
1. J Biomol NMR. 2012 Apr;52(4):329-37 - PubMed
1. Nat Rev Mol Cell Biol. 2015 Jan;16(1):18-29 - PubMed

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¹³C APSY-NMR for sequential assignment of intrinsically disordered proteins

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