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. 2018 Mar;70(3):167-175.
doi: 10.1007/s10858-018-0167-4. Epub 2018 Feb 28.

13C APSY-NMR for sequential assignment of intrinsically disordered proteins

Maria Grazia Murrali  1 , Marco Schiavina  1 , Valerio Sainati  1 , Wolfgang Bermel  2 , Roberta Pierattelli  3   4 , Isabella C Felli  5   6
Affiliations

13C APSY-NMR for sequential assignment of intrinsically disordered proteins

Maria Grazia Murrali et al. J Biomol NMR. 2018 Mar.

Abstract

The increasingly recognized biological relevance of intrinsically disordered proteins requires a continuous expansion of the tools for their characterization via NMR spectroscopy, the only technique so far able to provide atomic-resolution information on these highly mobile macromolecules. Here we present the implementation of projection spectroscopy in 13C-direct detected NMR experiments to achieve the sequence specific assignment of IDPs. The approach was used to obtain the complete backbone assignment at high temperature of α-synuclein, a paradigmatic intrinsically disordered protein.

Keywords: 13C detection; Assignment; IDPs; Intrinsically disordered proteins; NUS.

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