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. 2018 Feb 26;23(3):513.
doi: 10.3390/molecules23030513.

Black Tea Samples Origin Discrimination Using Analytical Investigations of Secondary Metabolites, Antiradical Scavenging Activity and Chemometric Approach

Wojciech Koch  1 , Wirginia Kukula-Koch  2 , Łukasz Komsta  3
Affiliations

Black Tea Samples Origin Discrimination Using Analytical Investigations of Secondary Metabolites, Antiradical Scavenging Activity and Chemometric Approach

Wojciech Koch et al. Molecules. .

Erratum in

Abstract

A comprehensive study on the composition and antioxidant properties of black tea samples with a chemometric approach was performed via LC-ESI-Q-TOF-MS, DPPH radical scavenging assay, and Folin-Ciocalteu assay (TPC). Marked differences between the teas from seven different countries (China, India, Iran, Japan, Kenya, Nepal, Sri Lanka) were shown. The Indian samples demonstrated the highest total catechin content (184.8 mg/100 mL), the largest TPC and DPPH scavenging potential (58.2 mg/100 mL and 84.5%, respectively). The applied principal component analysis (PCA) and ANOVA revealed several correlations between the level of catechins in tea infusions. EC (epicatechin), ECG (epicatechin gallate), EGC (epigallocatechin), and EGCG (epigallocatechin-3-gallate) content was not correlated with DPPH, gallic acid, and TPC; however, a strong correlation of EC and ECG between themselves and a negative correlation of these two catechins with EGCG and EGC was noted. Interestingly, simple catechins were not found to be responsible for antioxidant properties of the black teas. The samples collected in the higher altitudes were similar.

Keywords: Camellia sinensis; LC-ESI-Q-TOF-MS; antioxidant activity; black teas; catechins; principal component analysis.

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Conflict of interest statement

The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.

Figures

Figure 1
Figure 1
The scores of first two principal components of PCA analysis, explaining together 80% of information in the obtained dataset.
Figure 2
Figure 2
The loadings of first two components depicted on Figure 2. For explanations, see text.
Figure 3
Figure 3
The EIC chromatograms of all catechins identified in the extracts in the negative mode of LC ESI-Q-TOF-MS analysis, identified based on the comparison with reference compounds.
See this image and copyright information in PMC

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