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. 2018 Nov;59(4):e258-e261.
doi: 10.1111/ajd.12801. Epub 2018 Mar 2.

Activation of RhoA, Smad2, c-Src, PKC-βII/δ and JNK in atopic dermatitis

Jianyun Lu  1 , Rong Yin  2   3 , Zhibing Fu  1   2 , Veronica M Lee  2 , John Stuart Nelson  2   4 , Wenbin Tan  2
Affiliations

Activation of RhoA, Smad2, c-Src, PKC-βII/δ and JNK in atopic dermatitis

Jianyun Lu et al. Australas J Dermatol. 2018 Nov.

Abstract

Atopic dermatitis is a multifactorial skin disease characterised by chronic and relapsing inflammation whose pathogenesis is incompletely understood. We found that the expression of TGFβR1 and the activation of SMAD2, RhoA, JNK, PKC-βII/δ and c-Src were upregulated in the infiltrated inflammatory cells, fibroblasts and vasculatures in the dermis and epidermis. In addition, increases in the expression of TGFβR1 and phosphorylation levels of JNK and c-Src were positively correlated with the inflammatory progression of atopic dermatitis severity.

Keywords: JNK; PKC; RhoA; SMAD2; atopic dermatitis; c-Src; inflammation.

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Conflict of interest statement

Conflict of Interest: Disclosure: None Declared

Figures

Figure 1
Figure 1
Upregulation of TGFβR1, p-RhoA, p-Smad2, p-c-Src, p-PKC-βII/δ and p-JNK in AD lesions. The expression of TGFβR1 (a, b), levels of p-RhoA (c, d), p-Smad2 (e, f), p-c-Src (g, h), p-PKC-βII/δ (i, j) and JNK (k, l) were determined by IHC in AD lesions as compared to normal skins. The red inset in (h) is the higher magnification from the blue boxed area from the same image. The red inset in (j) shows p-PKC-βII/δ -IR Langerhans cells (indicated by purple arrows) in epidermis. Scar bar: 50 μm.
Figure 2
Figure 2
(a) Relative IHC scores of TGFβR1, p-RhoA, p-Smad2, p-c-Src, p-PKC-βII/δ and p-JNK in AD epidermis and dermal infiltrates. # p<0.05 as compared to normal skins. (b)-(e), The correlations of IHC scores of TGFβR1 in epidermis (b) and inflammatory infiltrate (c), p-c-Src (d) and p-JNK (e) in dermal infiltrated cells with patients’ SCORAD.
See this image and copyright information in PMC

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