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. 2018 Mar 7;10(3):318.
doi: 10.3390/nu10030318.

Selenium Accumulation, Antioxidant Enzyme Levels, and Amino Acids Composition in Chinese Mitten Crab (Eriocheir sinensis) Fed Selenium-Biofortified Corn

Linxi Yuan  1 , Ru Zhang  2   3 , Xuzhou Ma  4 , Ling Yang  5 , Qing Zheng  6 , Dong Chen  7 , Miao Li  8 , Ting Fan  9 , Yongxian Liu  10 , Liping Pan  11 , Xuebin Yin  12
Affiliations

Selenium Accumulation, Antioxidant Enzyme Levels, and Amino Acids Composition in Chinese Mitten Crab (Eriocheir sinensis) Fed Selenium-Biofortified Corn

Linxi Yuan et al. Nutrients. .

Abstract

The effects of selenium (Se)-biofortified corn on the total Se contents, the antioxidant enzyme levels, and the amino acids composition in Chinese mitten crab (Eriocheir sinensis) during the stage of the fifth shelling to maturity were investigated in the present study. The culture density of crabs was 600 per 667 m², and they were continuously fed 120.4 mg Se from Se-biofortified corn per 667 m² every two days for 90 days. The results showed that the total muscle Se levels in the crabs were significantly increased (p < 0.05). Activities of hemolymph supernatant enzymes including alkaline phosphatase (AKP), lysozyme (LZM), glutathione peroxidase (GPx), and superoxide dismutase (SOD) were also enhanced (p < 0.05). The protein and crude fat levels at maturity were higher than those at the fourth molt. The levels of total essential amino acids (∑EAAs) and total delicious amino acids (∑DAAs) were significantly increased (p < 0.05). We demonstrate that the feeding of Se-biofortified corn could significantly improve total muscle Se concentrations and hemolymph supernatant antioxidant enzyme activities in Chinese mitten crab, and slow down the rapid decline of ∑EAAs and ∑DAAs at maturity, thus improving the nutritional quality of Chinese mitten crab.

Keywords: Chinese mitten crab; Se-biofortified corn; amino acids content; antioxidant levels; hemolymph supernatant; muscle Se level.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The total selenium levels in crabmeat samples. Note: Data of columns marked with the same letter are not statistically different (p > 0.05). CK: samples on 29 July 2015 right after the fourth shelling; August: samples on 30 August 2015 right after the fifth shelling; September: samples on 30 September 2015 at the harvest stage.
Figure 2
Figure 2
Activities of hemolymph supernatant antioxidant enzymes. Note: Data of columns marked with the same letter are not statistically different (p > 0.05). CK: samples on 29 July 2015 right after the fourth shelling; August: samples on 30 August 2015 right after the fifth shelling; September: samples on 30 September 2015 at the harvest stage.
Figure 3
Figure 3
The correlation of the total Se levels in crabmeats and the activities of the hemolymph supernatant enzymes.
Figure 4
Figure 4
The correlation of the total Se levels in crabmeat and the protein contents, the fat levels, and the amino acid levels.
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