Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Mar 9;11(1):162.
doi: 10.1186/s13071-018-2633-y.

Survey of Anaplasma phagocytophilum and Anaplasma sp. 'Omatjenne' infection in cattle in Africa with special reference to Ethiopia

Sori Teshale  1   2   3 , Dirk Geysen  4 , Gobena Ameni  5 , Pierre Dorny  4   6 , Dirk Berkvens  4   6
Affiliations

Survey of Anaplasma phagocytophilum and Anaplasma sp. 'Omatjenne' infection in cattle in Africa with special reference to Ethiopia

Sori Teshale et al. Parasit Vectors. .

Abstract

Background: As evidence of the infection of domestic animals by Anaplasma phagocytophilum and Anaplasma sp. 'Omatjenne' is presently becoming available, understanding the epidemiological and ecological significance of infection is important to quantify the clinical and socio-economic impact of the diseases they cause.

Methods: The first aim of this study was to analyse the occurrence of A. phagocytophilum and Anaplasma sp. 'Omatjenne' in cattle samples collected from selected African countries using a polymerase chain reaction and restriction enzyme fragment length polymorphism. Secondly, this study was aimed at the molecular identification of Ehrlichia spp. and Anaplasma spp. infection in ruminants raised under different production systems in selected sites in central Ethiopia.

Results: In total, 695 samples from cattle in six African countries were analysed. Overall, 45 positive results were obtained for Anaplasma sp. 'Omatjenne' (6.47%) and 19 for A. phagocytophilum (2.73%). Anaplasma sp. 'Omatjenne' was detected in all countries except Tanzania while A. phagocytophilum was detected only in samples from Ethiopia. The proportion of samples tested positive for Anaplasma sp. 'Omatjenne' ranged from 1.2% in Morocco to 16% in Rwanda. The occurrence of both agents is now confirmed in African cattle. For the survey in Ethiopia a semi-nested 16S rDNA polymerase chain reaction followed by restriction fragment length polymorphism was used for the identification of Ehrlichia spp. and Anaplasma spp. in blood samples. Randomly selected samples were also analysed by pCS20 polymerase chain reaction for the detection of E. ruminantium. Positive results were obtained for E. ruminantium and five species of Anaplasma including a zoonotic species. To our knowledge, this is the first report of infection of domestic ruminants with A. phagocytophilum, A. ovis and Anaplasma sp. 'Omatjenne' in Ethiopia.

Conclusion: The present study showed widespread occurrence of Anaplasma sp. 'Omatijenne' in African cattle and five Anaplasma species in Ethiopia.

Keywords: Africa; Anaplasma phagocytophilum; Anaplasma sp.; Cattle; Ethiopia; ‘Omatjenne’.

PubMed Disclaimer

Conflict of interest statement

Ethics approval and consent to participate

Ethical clearance was obtained from the Animal Research Ethics Review Committee of the College of Veterinary Medicine and Agriculture of the Addis Ababa University. The clearance identification number is ARER/01/12/2012.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

References

    1. Dulmer JS, Barbet AF, CPJ B, Dasch GA, Palmer GH, Ray SC, et al. Reorganisation of genera in the families of Rickettsciaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrilichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettscia, descriptions of six new species combinations and designation of Ehrlichia equi and `HGE agent' as subjective synonyms of Ehrlichia phagocytophilia. Int J Syst Evol Biol. 2001;51:2145–2165. doi: 10.1099/00207713-51-6-2145. - DOI - PubMed
    1. Yoo-eam S. Molecular epidemiology of tick-borne Anaplasmataceae among client-owned dogs in Missouri. St. Louis: University of Missouri-Columbia; 2012.
    1. Stuen S, Granquist EG, Silaghi C. Anaplasma phagocytophilum - a widespread multi-host pathogen with highly adaptive strategies. Frontiers Cell Infect Microbiol. 2013;3:1127–1132. doi: 10.3389/fcimb.2013.00031. - DOI - PMC - PubMed
    1. Woldehiwet Z. The natural history of Anaplasma phagocytophilum. Vet Parasitol. 2010;167:108–122. doi: 10.1016/j.vetpar.2009.09.013. - DOI - PubMed
    1. Rymaszewska A, Grenda S. Bacteria Of the genus Anaplasma - characteristics of Anaplasma and their vectors: a review. Veterinarni Medicina. 2008;53:573–584. doi: 10.17221/1861-VETMED. - DOI

Publication types

MeSH terms