The Hidden Face of Rubisco

Abstract

Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) fixes atmospheric CO₂ into organic compounds and is composed of eight copies each of a large subunit (RbcL) and a small subunit (RbcS). Recent reports have revealed unusual RbcS, which are expressed in particular tissues and confer higher catalytic rate, lesser affinity for CO₂, and a more acidic profile of the activity versus pH. The resulting Rubisco was proposed to be adapted to a high CO₂ environment and recycle CO₂ generated by the metabolism. These RbcS belong to a cluster named T (for trichome), phylogenetically distant from cluster M, which gathers well-characterized RbcS expressed in mesophyll or bundle-sheath tissues. Cluster T is largely represented in different plant phyla, including pteridophytes and bryophytes, indicating an ancient origin.

Keywords: CO(2) recycling; RbcS; evolution; photosynthesis; structure; trichome.