Immunocytochemistry of cardiac polypeptide hormones (cardiodilatin/atrial natriuretic polypeptide) in brain and hearts of Myxine glutinosa (Cyclostomata)

Abstract

With the use of different region-specific antisera against partial sequences of porcine cardiodilatin (CDD)-126 and the peroxidase-antiperoxidase (PAP) technique, the central nervous system as well as the systemic and the portal vein heart of the cyclostomian species Myxine glutinosa were investigated for a possible existence of cardiac polypeptides. In contrast to mammals, CDD-immunoreactions were obtained only with antisera directed against the C-terminus of CDD (CDD 99-126) which is identical to human atrial natriuretic polypeptide (alpha hANP). CDD-immunoreactive myocardiocytes were found in high densities in the atrium of the systemic heart and in the portal vein heart. In the ventricle of the systemic heart, CDD-immunoreactive cells were extremely scarce. In agreement with the immunohistochemical results, myoendocrine cells analyzed by electronmicroscopy exhibited specific granules of an average diameter of 0.21 + 0.02 micron in equivalent localizations. Furthermore, with the use of the protein A-gold (PAG) technique, CDD-immunoreactivity was ultrastructurally localized within the specific granules of atrial myocardiocytes. In the central nervous system of Myxine glutinosa, CDD-immunoreactive perikarya and/or fibers were present on all levels from the telencephalon to the spinal cord. The results of the present study are compared with those obtained in mammals and their possible functional relevance and their meaning in phylogeny are discussed as well.