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. 2018 Feb;56(1):71-74.
doi: 10.3347/kjp.2018.56.1.71. Epub 2018 Feb 28.

Serodiagnosis of Extraintestinal Amebiasis: Retrospective Evaluation of the Diagnostic Performance of the Bordier® ELISA Kit

Nicolas Beyls  1   2 , Odile Cognet  2 , Jean-Paul Stahl  3 , Olivier Rogeaux  4 , Herve Pelloux  2
Affiliations

Serodiagnosis of Extraintestinal Amebiasis: Retrospective Evaluation of the Diagnostic Performance of the Bordier® ELISA Kit

Nicolas Beyls et al. Korean J Parasitol. 2018 Feb.

Abstract

Soluble antigens from an axenic culture of Entamoeba histolytica were used to develop a commercial ELISA kit to quantify anti-E. histolytica antibodies in sera of patients with extraintestinal amebiasis in non-endemic settings. The diagnostic specificity and sensitivity of the test were assessed retrospectively using 131 human serum samples with amoebic serologic status available. They were selected according to their results in immunofluorescence (IFAT) and were separated in 2 sample categories: 64 sera with positive results by IFAT and 67 with negative results by IFAT. The sensitivity and specificity of the ELISA kit were assessed at 95.0% and 94.0% compared to the IFAT. The test can be useful to exclude a potential diagnosis of amebiasis and could be used as a screening method since ELISA is an automated technique.

Keywords: ELISA; Entamoeba histolytica; amebiasis; diagnosis.

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Conflict of interest statement

N. Beyls is employed by the company Bordier Affinity Products SA.

References

    1. Tanyuksel M, Petri WA., Jr Laboratory diagnosis of amebiasis. Clin Microbiol Rev. 2003;16:713–729. - PMC - PubMed
    1. Ali IK, Clark CG, Petri WA., Jr Molecular epidemiology of amebiasis. Infect Genet Evol. 2008;8:698–707. - PMC - PubMed
    1. Mortimer L, Chadee K. The immunopathogenesis of Entamoeba histolytica . Exp Parasitol. 2010;126:366–380. - PubMed
    1. Kimura K, Stoopen M, Reeder MM, Moncada R. Amebiasis: modern diagnostic imaging with pathological and clinical correlation. Semin Roentgenol. 1997;32:250–275. - PubMed
    1. Merens A, Rapp C, Fabre R, Cavallo JD. Utility and limitations of laboratory diagnosis of amebiasis. Med Trop. 2005;65:167–175. (in French) - PubMed

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