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. 2018 Mar 13;15(1):44.
doi: 10.1186/s12985-018-0951-5.

Comparison of two different combined test strips with fluorescent microspheres or colored microspheres as tracers for rotavirus and adenovirus detection

Na Jiang  1 , Lei Shi  1 , Jieping Lin  1 , Lifang Zhang  1 , Yanxia Peng  1 , Huiying Sheng  2 , Ping Wu  3 , Qingjun Pan  4
Affiliations

Comparison of two different combined test strips with fluorescent microspheres or colored microspheres as tracers for rotavirus and adenovirus detection

Na Jiang et al. Virol J. .

Abstract

Background: Rotavirus (RV) and enteric adenovirus (AdV) mainly cause infantile infectious gastroenteritis. Several separate test methods for the detection of RV or AdV are currently available, but few tests are able to simultaneously detect both RV and AdV viruses, especially in primary medical institutions.

Methods: The present study was mainly designed to compare the performance of two combined test strips for the detection of RV and AdV: a rotavirus-adenovirus strip with fluorescent microspheres for tracers (FMT); and the CerTest rotavirus-adenovirus blister strip with colored microspheres for tracers (CMT). To test the strips cultures of RV, AdV and from other enteric pathogens were used, in addition to 350 stool specimens from 45 symptomatic patients with gastrointestinal infections.

Results: Detection thresholds for RV and AdV cultures using serial dilutions showed that the sensitivity of FMT was significantly higher than that of CMT (both P < 0.05). Specificity evaluation demonstrated that with culture mixtures of Coxsackie (A16), ECHO (type30), and entero- (EV71) viruses there was no detection of cross reaction using the two test strips, i.e., all the results were negative. With regard to the detection of RV in 350 clinical specimens, the total coincidence rate was 92.9%, the positive coincidence rate was 98.2%, and the negative coincidence rate was 90.8%. With regard to AdV detection, the total coincidence rate was 95.4%, the positive coincidence rate was 95.2%, and the negative coincidence rate was 95.5%.

Conclusions: FMT performed better than CMT with regard to the combined detection of RV and AdV.

Keywords: Adenovirus; Colored microspheres; Fluorescent microspheres; Rotavirus; Sensitivity; Specificity.

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Conflict of interest statement

Ethics approval and consent to participate

Procedures were reviewed and approved by the Committee of the Affiliated Hospital of Guangdong Medical University.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

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Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
Test modes of the two combined detection test strips. Fluorescent microspheres for tracers (FMT): Control line (C); Detection line (T) including the enteric adenovirus (AdV) line (near the sample loading well) and the rotavirus (RV) line. Interpretation of results: after the test procedures, double positive (left) and negative (right) results were detected. Colored microspheres for tracers (CMT): Control line (blue); Detection line (red) including the AdV line (near the sample loading end) and the RV line. Interpretation of results: after the test procedures, double positive (left) and negative (right) results were detected
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