Effect of Shallow and Deep SCUBA Dives on Heart Rate Variability

Abstract

Prolonged and high pressure diving may lead to various physiological changes including significant alterations of autonomic nervous system (ANS) activity that may be associated with altered physical performance, decompression sickness, or central nervous system oxygen toxicity. Ideally, researchers could elucidate ANS function before, during, and after dives that are most associated with altered function and adverse outcomes. However, we have a limited understanding of the activities of the ANS especially during deeper prolonged SCUBA diving because there has never been a convenient way to collect physiological data during deep dives. This work is one of the first studies which was able to collect electrocardiogram (ECG) data from SCUBA divers at various depths (33, 66, 99, 150, and 200 ftsw; equivalent to 10.05, 20.10, 30.17, 45.72, and 60.96 m of salt water, respectively) breathing different gas mixtures (air, nitrox and trimix). The aim of this study was to shed light on cardiac ANS behavior during dives, including deep dives. With the aid of dry suits, a Holter monitor that could handle the pressure of a 200 ft. dive, and a novel algorithm that can provide a useful assessment of the ANS from the ECG signal, we investigated the effects of SCUBA dives with different time durations, depths and gas mixtures on the ANS. Principal dynamic mode (PDM) analysis of the ECG, which has been shown to provide accurate separation of the sympathetic and parasympathetic dynamics, was employed to assess the difference of ANS behavior between baseline and diving conditions of varying depths and gas mixtures consisting of air, nitrox and trimix. For all depths and gas mixtures, we found consistent dominance in the parasympathetic activity and a concomitant increase of the parasympathetic dynamics with increasing diving duration and depth. For 33 and 66 ft. dives, we consistently found significant decreases in heart rates (HR) and concomitant increases in parasympathetic activities as estimated via the PDM and root mean square of successive differences (RMSSD) for all time intervals (from the first 5 min to the last 30 min) at the bottom depth when compared to the baseline depth at sea level. The sympathetic dynamics did not change with dive duration or gas mixtures, but at the 150 and 200 ft. dives, we found a significant increase in the sympathetic dynamics in addition to the elevated parasympathetic dynamics when compared to baseline The power spectral density (PSD) measures such as the low frequency (LF), high frequency (HF) and its ratio, and approximate entropy (ApEn) indices were not as consistent when compared to PDM-derived parasympathetic dynamics and RMSSD index.

Keywords: SCUBA; autonomic nervous system; decompression; heart rate variability; nitrox; principal dynamic mode; trimix.

Figure 1

Typical dive profiles of the 33 ft. (Top) and 66 ft. (Bottom) dives, respectively.

Figure 2

Depth comparison of HRV parameters. The line marked with diamonds represents baseline; the square line is bottom condition. + means p < 0.05 when comparing the bottom to its corresponding baseline by paired t-test. *denotes p < 0.05 when comparing to the 33 ft. condition. Error bars denote standard error.

Figure 3

Effects of breathing gases on HRV parameters. Darker bars represent the baseline and the gray bars represent the bottom condition (99 ft.). + denotes p < 0.05 when comparing the bottom to the corresponding baseline. Error bars denote standard error.