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. 2018 Mar 13;12(1):27.
doi: 10.1186/s13065-018-0397-2.

Optimized method for determination of 16 FDA polycyclic aromatic hydrocarbons (PAHs) in mainstream cigarette smoke by gas chromatography-mass spectrometry

Jana Jeffery  1 , Maria Carradus  2 , Karolina Songin  2 , Michael Pettit  2 , Karl Pettit  2 , Christopher Wright  3
Affiliations

Optimized method for determination of 16 FDA polycyclic aromatic hydrocarbons (PAHs) in mainstream cigarette smoke by gas chromatography-mass spectrometry

Jana Jeffery et al. Chem Cent J. .

Abstract

A gas chromatography-mass spectrometry (GC-MS) method was validated for the determination of 16 polycyclic aromatic hydrocarbons (PAHs) from the FDA list of 93 harmful or potentially harmful constituents of mainstream cigarette smoke (MCS). Target analytes were extracted from total particulate matter using accelerated solvent extraction with a toluene/ethanol solvent mixture. Matrix artefacts were removed by two-step solid-phase extraction process. Three different GC-MS systems [GC-MS (single quadrupole), GC-MS/MS (triple quadrupole) and GC-HRMS (high resolution, magnetic sector)] using the same separation conditions were compared for the analysis of MCS of 3R4F Kentucky reference cigarettes generated under ISO and intense smoking regimes. The high mass resolution (m/∆m ≥ 10,000) and associated selectivity of detection by GC-HRMS provided the highest quality data for the target PAHs in MCS. Owing to the HR data acquisition mode enabling measurement of accurate mass, limits of quantification for PAHs were 5 to 15-fold lower for GC-HRMS than for GC-MS/MS and GC-MS. The presented study illustrates that the optimised sample preparation strategy followed by GC-HRMS analysis provides a fit-for-purpose and robust analytical approach allowing measurement of PAHs at (ultra)low concentrations in MCS. Furthermore, the study illustrates the importance and benefits of robust sample preparation and clean-up to compensate for limited selectivity when low-resolution MS is used.

Keywords: Accelerated solvent extraction; Gas chromatography–mass spectrometry; High resolution mass spectrometry; Low resolution mass spectrometry; Mainstream cigarette smoke; Polycyclic aromatic hydrocarbons (PAHs).

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Figures

Fig. 1
Fig. 1
Flow chart of analytical procedure
Fig. 2
Fig. 2
Benzo[b]fluoranthene and B[a]P separation and sensitivity (S/N) on tested GC/MS systems in 3R4F ISO MCS
Fig. 3
Fig. 3
PAHs in 3R4F ISO MCS (a). Zoom view PAHs at (ultra)low levels (b)
See this image and copyright information in PMC

References

    1. Ingebrethsen BJ. Aerosol studies of cigarette smoke. Recent Adv Tob Sci. 1986;12:54–142.
    1. Rodgman A, Perfetti TA. The chemical components of tobacco and tobacco smoke. 2. Boca Raton: CRC Press; 2013.
    1. Fowles J, Dybing E. Application of toxicological risk assessment principles to the chemical constituents of cigarette smoke. Tob Control. 2003;12(4):424–430. doi: 10.1136/tc.12.4.424. - DOI - PMC - PubMed
    1. Bentley HR, Burgan JG. Polynuclear hydrocarbons in tobacco and tobacco smoke. Part II. The origin of 3:4-benzopyrene found in tobacco and tobacco smoke. Analyst. 1960;85:723–727. doi: 10.1039/an9608500723. - DOI
    1. Hoffmann D, Adams JD, Lisk D, Fisenne I, Brunnemann KD. Toxic and carcinogenic agents in dry and moist snuff toxic and carcinogenic agents in dry and moist snuff. J Natl Cancer Inst. 1987;79:1281–1286. - PubMed