Single-Cell Protein Assays: A Review

Beiyuan Fan^{1

2}, Junbo Wang^{3

4}, Ying Xu⁵, Jian Chen^{6

7}

Affiliations

¹ State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing, 100190, China.
² University of Chinese Academy of Sciences, Beijing, 100049, China.
³ State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing, 100190, China. jbwang@mail.ie.ac.cn.
⁴ University of Chinese Academy of Sciences, Beijing, 100049, China. jbwang@mail.ie.ac.cn.
⁵ Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of Ministry of Education, Shanghai Jiao-Tong University School of Medicine, Shanghai, 200025, China. yingxuxu@shsmu.edu.cn.
⁶ State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing, 100190, China. chenjian@mail.ie.ac.cn.
⁷ University of Chinese Academy of Sciences, Beijing, 100049, China. chenjian@mail.ie.ac.cn.

PMID: 29536450
DOI: 10.1007/978-1-4939-7717-8_17

Review

Single-Cell Protein Assays: A Review

Beiyuan Fan et al. Methods Mol Biol. 2018.

. 2018:1754:293-309.

doi: 10.1007/978-1-4939-7717-8_17.

Authors

Beiyuan Fan^{1

2}, Junbo Wang^{3

4}, Ying Xu⁵, Jian Chen^{6

7}

Affiliations

¹ State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing, 100190, China.
² University of Chinese Academy of Sciences, Beijing, 100049, China.
³ State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing, 100190, China. jbwang@mail.ie.ac.cn.
⁴ University of Chinese Academy of Sciences, Beijing, 100049, China. jbwang@mail.ie.ac.cn.
⁵ Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of Ministry of Education, Shanghai Jiao-Tong University School of Medicine, Shanghai, 200025, China. yingxuxu@shsmu.edu.cn.
⁶ State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing, 100190, China. chenjian@mail.ie.ac.cn.
⁷ University of Chinese Academy of Sciences, Beijing, 100049, China. chenjian@mail.ie.ac.cn.

PMID: 29536450
DOI: 10.1007/978-1-4939-7717-8_17

Abstract

Quantification of single-cell proteomics provides key insights in the field of cellular heterogeneity. This chapter discusses the emerging techniques that are being used to measure the protein copy numbers at the single-cell level, which includes flow cytometry, mass cytometry, droplet cytometry, microengraving, and single-cell barcoding microchip. The advantages and limitations of each technique are compared, and future research opportunities are highlighted.

Keywords: Droplet cytometry; Flow cytometry; Mass cytometry; Microengraving; Single-cell barcoding microchip; Single-cell protein assays.

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Single-Cell Protein Assays: A Review

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Single-Cell Protein Assays: A Review

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