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. 2018 Jun 4;19(2):87-93.
doi: 10.4274/jtgga.2017.0047. Epub 2018 Mar 16.

Does progesterone have protective effects on ovarian ischemia-reperfusion injury?

Banu Güleç Başer  1 , Mine İslimye Taşkın  1 , Ertan Adalı  1 , Emine Öztürk  1 , Adnan Adil Hısmıoğulları  2 , Arzu Yay  3
Affiliations

Does progesterone have protective effects on ovarian ischemia-reperfusion injury?

Banu Güleç Başer et al. J Turk Ger Gynecol Assoc. .

Abstract

Objective: The aim of the present study was to evaluate the effects of progesterone (PG) against ovarian ischemia-reperfusion (I/R) injury through the evaluation of biochemical and histopathologic parameters.

Material and methods: Twenty-one female Wistar albino rats were divided into three groups. Group 1: Sham; group 2: I/R; group 3: I/R+PG (8 mg/kg). PG was administered intraperitoneally to the rats in group 3, 30 minutes before a detorsion operation. Ovarian I/R injury was evaluated in serum and tissue by using biochemical parameters including malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index, neutrophil gelatinase-associated lipocalin (NGAL) and immunofluorescence staining by using a terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay.

Results: Serum and tissue TOS levels were significantly lower in group 3 than in group 2. Tissue TAS levels were higher in group 3 than in group 2 (p<0.001). NGAL and MDA levels were similar between the groups. Histologic score, including vascular congestion, hemorrhage, polymorphonuclear neutrophils, and interstitial edema, was higher in group 2. Pre-treatment with PG decreased the score, but this difference was not statistically significant. The number of apoptotic cells was higher in group 2 than in groups 1 and 3. The TUNEL-positive cell number decreased with PG in group 3.

Conclusion: Preoperative PG treatment might exert protective effects on ovarian I/R injury through its anti-apoptotic and antioxidative properties.

Keywords: Ischemia-reperfusion; NGAL; progesterone.

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Conflict of interest statement

Conflict of Interest: No conflict of interest is declared by the authors.

Figures

Figure 1
Figure 1. Apoptotic cells are stained with terminal deoxynucleotidyl TUNEL in groups. TUNEL-positive cells reflective green immunofluorescence. Positive apoptotic cells were counterstained with DAPI nuclear staining. There were many apoptotic cells in group 2. There were only a few TUNEL-positive cells in the ovaries of group 3 (Original magnification, 400x) DAPI: 4',6-diamidino-2-phenylindole, TUNEL: Transferase-mediated dUTP nick end labeling
Figure 2
Figure 2. Light microscopic findings of the groups (100x, Original magnification, Masson trichrome). Ovarian sections in group 2 (I/R) showed severe damage, infiltration of polymorphonuclear leukocytes, vascular congestion, interstitial edema*, hemorrhage → → I/R: Ischemia/reperfusion
Graphic 1
Graphic 1. Histopathologic score in groups I/R: Ischemia/reperfusion, PG: Progesterone
See this image and copyright information in PMC

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References

    1. Ipek A, Tan S, Kurt A, Yesilkaya Y, Orhan D. Prenatal over kisti torsiyonu: US ve MR görüntüleme bulguları. Türkiye Klinikleri J Gynecol Obst. 2010;20:262–5.
    1. Maretta M, Toth SS, Bujdos M, Toth S Jr, Jonecova Z, Vesela J. Alterations of epithelial layer after ischemic preconditioning of small intestine in rats. J Mol Histol. 2012;43:171–8. - PubMed
    1. Wang J, Jiang C, Liu C, Li X, Chen N, Hao Y. Neuroprotective effects of progesterone following stroke in aged rats. Behav Brain Res. 2010;209:119–22. - PubMed
    1. Allen RS, Olsen TW, Sayeed I, Cale HA, Morrison KC, Oumarbaeva Y, et al. Progesterone treatment in two rat models of ocular ischemia. Invest Ophthalmol Vis Sci. 2015;56:2880–91. - PMC - PubMed
    1. Morrissy S, Xu B, Aguilar D, Zhang J, Chen QM. Inhibition of apoptosis by progesterone in cardiomyocytes. Aging Cell. 2010;9:799–809. - PMC - PubMed

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